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Production, Partially Purification and Estimation of serratiopeptidase from serratia marcescens | ||
| Al-Mustansiriyah Journal of Science | ||
| Article 1, Volume 27, Issue 1, 2016, Pages 33-35 | ||
| Author | ||
| Manal Khalid | ||
| Abstract | ||
| Production, Partially Purification and Estimation of serratiopeptidase from serratia marcescens was the base of this study .Serratiopeptidase (STP) is a proteolytic enzyme with wide medical applications .Serratia marcescens strain was cultured in production media (PM) containing maltoseas carbon source and the nitrogen source used were soy bean meal. The Serratiopeptidase (STP) enzyme activity was determined according to gelatin clearing zone .Optimal condition for (STP) enzyme productionin Optimal temperature 32C0was 25hrs Incubation period; media ingredient was glucose and pH= 7.3.The crud enzyme production gave (0.7) Relative enzyme activity (REA)at 68th hrs.At partially purification in 40% saturationt by ammonium sulphate gave (0.73) Relative enzyme activity REA. While dialysed sample gave (0.76)Relative enzyme activity REA. From results it is concluded that Serratiopeptidase activity increased as the increase in enzyme concentration also STP enzyme production affected by the experiment condition. | ||
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