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The Qualification and Quantification of Caffeine in Two Different Caffeinated Pharmaceutical Formulas Employing RP-HPLC Technique. | ||
| Albahir journal | ||
| Article 1, Volume 2, Issue 3, 2015, Pages 76-89 | ||
| Abstract | ||
| Abstract Background: Method optimization of drug analysis is an arising approach among multidisciplinary science fields. The High performance liquid chromatography (HPLC) has been approved to be the cornerstone of drug analysis. Over the last ten years, HPLC has been figured as the analysis method of choice for many compounds. HPLC system has been utilized in diversified applications such as: the quantitative/qualitative analyses of biological samples, calculating the amounts of active pharmaceutical ingredient (API), isolating specific components from their mixtures, and many others. This work aims to qualify and quantify the caffeine in two different pharmaceutical formulas namely; Panadol® Extra tablets and Thicker Fuller Hair® shampoo employing RP-HPLC. Methods: Isocratic method was utilized with fixed mobile phase ratio as methanol: water (40:60). The flow rate was 0.8 ml/min while the wavelength was 273nm. Calibration curve was plotted via the utilization of the following concentrations (0.0, 20, 40, 60, and 80) µg/ml. Thirty six caffeinated tablet samples were examined as dissolved by methanol and filtered with 0.22 µm nylon-membrane syringe filter and diluted. On the other hand three independent caffeinated shampoo samples were examined. The samples were diluted, shacked, and filtered with 0.22 µm nylon-membrane syringe filters. Finally, the diluted solutions were injected to the RP-HPLC and the data was observed and recorded. Results: The areas required to plot the calibration curve were observed. The caffeine peak was identified within each pharmaceutical sample. Consequently the identified peaks were also quantified. The mean content of caffeine in caffeinated tablets was found to be 64.96 mg. Moreover, the caffeine concentration in the shampoo was observed as 0.0047%. Discussion and conclusion: In this work the RP-HPLC has been utilized in the qualification and quantification of caffeine in two different pharmaceutical formulas namely; Panadol® Extra tablets and Thicker Fuller Hair® shampoo. HPLC technology has matured to the extent that almost any existing drug can be analyzed by an existing method that can be found in the analytical literatures. For future work other drugs can be examined or even multidrug formulas can be tried with such accurate and precise RP-HPLC technique. | ||
| Keywords | ||
| Caffeine; HPLC; Panadol Extra Tablets; Thicker Fuller Caffeinated Shampoo | ||
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