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GOAT OOCYTES MATURATION , FERTILIZATION AND CLEAVAGE IN VITRO WITH THE ADDITION OF THREE DIFFERENT HORMONES | ||
| The Iraqi Journal of Agricultural Science | ||
| Article 1, Volume 36, Issue 3, 2005, Pages 163-168 | ||
| Abstract | ||
| ABSTRACT Goat ovaries were collected from the abattoir (30 km) near Baghdad. 20 minutes after slaughtering, 2276 goat oocytes were used in this study which collected from these ovaries, (1321) oocytes were collected from the ovaries insulated in cold at (0° C), and 995 oocytes collected from warm ovaries at (- 30°C). Each group was divided into three subgroups and started their maturation in TCM-199 media with addition one of the three hormonal treatments (Metrodin, gonal -F and pergonal). These oocytes in each subgroup were redivided into with and without cumulus cells. After 27 h of maturation at 38° C with 5% CO2 and 95/0 humidity, they were fertilized by a capacitated fresh semen of fertile bucks, then reincubated at the same conditions mentioned above for 24 hours to find the cleavage of zygotes. Results revealed a significant (P < O.OI) effect for the addition ofPergonal to the maturation media for oocytes with and without cumulus taken from cold ovaries and with Metrodin for the oocytes taken from the warm ovaries. Also there were a significant (P < 0 0 1) increase in maturation rate for the oocytes with cumulus compared to the oocytes without cumulus in both groups of cold and warm ovaries. The highest fertilization percentage with high significance (P < 0.01) were noticed in the media with the addition Gonal -F in both with and without cumulus oocytes .. and the .group with cumulus cells were superior than without cumulus oocytes The cleavage percentage after fertilization were the highest in the oocyte taken from cold ovaries with the addition of Gonal -F, and Metrodin for the oocytes with cumulus taken from warm ovaries by a significant (P < 0.01) differences as compared with the other media studied. The oocytes without cumulus In all media were without cleavage, which maya cause: of the absence of epidermal growth factor in the maturation media who has the stimulation effect 011 cleavage. | ||
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