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Molecular identification of some Fungi caused Broad bean root and crown disease controlled by using Rhizobium leguminosarum biovar viciae bacteria and Bioagents Trichoderma viride in the province of Babylon | ||
| Euphrates Journal of Agriculture Science | ||
| Article 1, Volume 7, Issue 4, 2015, Pages 370-386 | ||
| Authors | ||
| Ahed A H Matloob; Mohamed A I K Alkaif | ||
| Abstract | ||
| The field survey results that conducted in 15 fields planted with Broad bean belong to Babylon province showed the spread of roots and bases of peas stems rot disease in all the regions that covered with this survey with infection percentage of about 40 – 100% with a severity of injury about 26.7 – 75%. The results of isolation and diagnosis Variation in the presence of fungi found that the fungus Rhizoctonia solani , Fusarium solani and Macrophomina phaseolina The most visible. The results of molecular diagnostics of these fungi proved that there are 12 isolates R. solani and 9 isolates of the fungus M. phaseolina complete except the fungus F. solani do not appear complete with its two isolates used by technical of PCR Polymerase Chain Reaction. The test results of the ability of pathogenic fungi studied showed Variation in the rates of germination and variation in the severity of infection. Been getting 7 isolates of bacteria Rhizobium leguminosarum biovar viciae of been has been getting and the results showed the ability of these isolates were discouraged by pathogenic fungi and ranged from 50.74-100%. The results of wooden canopy showed that the treatment outweigh the integration between bacteria and mushrooms R. leguminosarum bio T. viride added to the contaminated soil fungus pathogen causing significance reduction in the proportion of the severity of rot disease and the base of peas stems ranging between 53.33- 66.67% and 15.00-18.33% respectively as observed superiority in increasing the growth standards in the plant compared with comparison treatment of mushroom alone. | ||
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