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Bacteriological Study of Some Genus of Enterobacteriaceae causes diarrhea in Children | ||
| Diyala Journal For Pure Science | ||
| Article 1, Volume 12, Issue 4, 2016, Pages 1-13 | ||
| Authors | ||
| Raya Khaleel Ibrahim Al-Qaysi; Hadi Rahman Rasheed Al-Taai; Abbas Aboud Farhan Al-Dolaymi | ||
| Abstract | ||
| This study included Isolation and identification of Proteus mrabilis , Salmonella Spp and Morganella morganii Causes Diarrhea in (215) samples for patients sufferers symptoms of Diarrhea. Period from 1102014 to 3012015, and used MacConkey agar and blood agar for the cultivation of these samples. The results refer that 65 isolates are belonging to bacteria of Enterobacteriaceae 13 (20 %) Proteus mirabilis and 3(4.6%)Morganella morganii 2(3.1%) Salmonella Spp by using diagnostic phenotypic ,biochemical tests and confirm the diagnosis by using system Vitek2.investigation results of some virulence factors showed isolates of Proteus mirabilis produce of haemolysin (92.3%) ,while Salmonella Spp and Morganella morganii have not ability to produce this enzyme.The results showed that all isolates of Salmonella Spp and Morganella morganii were produced protease by (100%)(50%) while Proteus mirabilis have not ability to produce this enzyme. The results showed Isolates of Proteus mirabilis showed highest resistance rate (76.9%) for Ampicillin while isolates of Salmonella Spp highest resistance rate (100%) for Cephalothin, Cefotaxime and Ampicillin , while isolates of Morganella morganii showed highest resistance rate %100 for Trimethoprime , Ampicillin , Cephalothin , Nalidixic acid , Piperacilli . The results showed Isolates of Proteus mirabilis showed highest tolerate for (CO) metal (0.4)mml and to isolates Morganella morganii (0.5)mml and to isolates Salmonella spp (0.5) mml percentage (100%) for each Isolates. For (Cu) metal Isolates of Proteus mirabilis and Morganella morganii showed highest tolerate to (1)mml percentage (66.66%) and to isolates Salmonella spp showed highest tolerate(0.4) mml percentage (100%) . | ||
| Keywords | ||
| Enterobacteriacea; virulent factore; Antibiotics; Heavy Metals | ||
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