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Estimation of 5`-nucleotidase activity from saliva of diabetic patients with partial purification of its isoenzymes | ||
| Tikrit Journal of Pure Science | ||
| Article 1, Volume 20, Issue 2, 2015, Pages 105-109 | ||
| Authors | ||
| a Ismail Yaseen; | ||
| Abstract | ||
| This study was performed on (60) saliva specimens, including (30) diabetic patients in addition (30) healthy saliva specimens were taken from healthy subjects as control group. The results showed a significant increase in the activity of saliva 5`-nucleotidase (5`-NT) from diabetic patients compared to healthy subjects. This study included a partial purification of 5`-NT as well, using (35%) (NH4)2SO4 for precipitation and gel filteration by (sephadex G-75 ) which gave single peak. Then 5-NT was partially also purified by using Ion exchange chromatography ( DEAE-cellulos A-50), in which three isoenzymes were isolated with different degree of purity. | ||
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