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The effect of Auxin IAA and sea algae extraction on oil characteristics and active compounds of (Ocimiumbasilicum L.) | ||
| Tikrit Journal of Pure Science | ||
| Article 1, Volume 20, Issue 3, 2015, Pages 19-24 | ||
| Author | ||
| Ihsan Abdl Azez Abdul Raheem | ||
| Abstract | ||
| In present study, Auxin (IAA) and sea algae extraction were used on Ocimiumbasilicum L. in a field in Tikrit University on 2013.Two factors were applied: A0 without spraying Auxin and A1spraying with 50 mg/L (IAA). The second factor was spraying sea algae extraction (Kelpak) in fourlevels: E0 without spraying, E1 spray of 1.5ml/L of sea algae (Kelpak), and E2 spray with 1.5 ml/L sea algae (ultrakelpa40), E3 spray 1.5+1.5ml/L mixed of sea algae (Kelpak and ultrakelpak40) together. The study designed by Randomised complete block design RCBD. Duncan’s test wasperformed to compare the medians of treatments with multi-limits at 5% possibility. The results showed: the treatments with 50mg/L of (IAA) and the combination treatment of 1.5+1.5 ml/L of sea algae (Kelpak and ultrakelpak40) caused a significant increase of physical properties of oil as follow: high level of specific gravity (0.902 gm/ml), high level of refractive index (1.252) and high level of oil concentration (1.021%)in compare with non treated plants with less level of specific gravity, refractive index and refractive index (0.876 gm/ml, 1.241, 0.74%) respectively. The results also showed that the treatment A1E1 significantly affected oil components and gave highest level of 2.5dimethoxybenzoic acid Ocimol(79.18), the treatment A0E3 caused highest level of Methyl eugenol (68.02), the treatment A0E2 gave highest level of Methycinammate (262.20) and Cubebene (159.88), the treatment A1E2 gave highest level of Linalool (204.99), the treatment A0E2 gave highest level of Geranyle acetate (342.61). The spraying with sea algae extract also affected those active substances as follow: the plants which treated with A1E1 gave the highest level of Caftaric acid (590.93) and Quercetin (213.17) in compare with the treatment of A1E3 that gavehighest level of Myriscetin (514.21) and highest level of both Kaempferol (916.27) and Anthraquimones (551.45), the treatment of A0E1 caused an increase in the concentrations of Rutine(477.12) and Quercetin (314.80) and Luteolin (285.09). | ||
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