Effect of Salicylic acid on the antioxidant enzymes activity for callus of Garnem peach rootstock under in vitro salt stress

Jalal Joudi, Zainab; Chellab Abbas, Muhson

	Effect of Salicylic acid on the antioxidant enzymes activity for callus of Garnem peach rootstock under in vitro salt stress
Kufa Journal for Agricultural Science
Article 1, Volume 8, Issue 4, 2016, Pages 22-36
Authors
Zainab Jalal Joudi; Muhson Chellab Abbas
Abstract
An experiment was conducted at the laboratory of plant tissue culture in the Department of Horticulture and Landscape at the College of Agriculture / University of Kufa to study the effect of salicylic acid onthe activity of antioxidant enzymes of Garnem callus rootstock under in vitro salt stress through of callus cultured on MS medium provided with (0, 30, 60, 120) mM of sodium chloride and (0, 0.1, 0.2, 0.3) mM of salicylic acid for 21 days. Result showed a significant effect ofsodium chloride salt on the activity of antioxidant enzymes Superoxide dismutase (SOD), Ascorbate peroxidase (APX) and Catalase (CAT) that recorded highest values at the concentration of 120mM compared with the control treatment recorded lowest values. Salicylic acid also has significant effect on the activity of antioxidant enzymes that increased in 0.3mM of salicylic acid except SOD enzymes activity which decreased at 0.3mM> Interaction between 120mM sodium chloride salt and 0.2mM salicylic acid gave highest values of antioxidant enzyme activity (SOD , APX) while 120mM sodium chloride salt and 0.3mM salicylic acid gave highest value of CAT enzymes activity compared with control treatment.
Keywords
Peach rootstock; Salt stress; Salicylic acid; Callus; Antioxidant enzyme activity

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