The relationship between sesame Phytoplasma (phyllody) and others plant Phytoplasma by using polymerase chain reaction (PCR)

Hussein Mohammed, Maytham; Abd Alhussein Alfadhal, Fadhal; Obais Alhatami, Abdullah

	The relationship between sesame Phytoplasma (phyllody) and others plant Phytoplasma by using polymerase chain reaction (PCR)
Kufa Journal for Agricultural Science
Article 1, Volume 8, Issue 4, 2016, Pages 130-150
Authors
Maytham Hussein Mohammed; Fadhal Abd Alhussein Alfadhal; Abdullah Obais Alhatami
Abstract
This study was aimed to identification of sesame phytoplasma (phyllody) on sesame (Sesame indicumL.) and some other important economic crops behind the weeds of agriculture field like Annual beard grass(Polypogon monspeliensis), Darnel (Lolium temulentum L.), Suaeda1 (Schanginia baccata MQ.T), Melilotus (Melilotus), Purple panic – grass (Echinochloa colonum), Cynanchum (Cynanchum acutum L.), Alkali weed (Cressa cretica L.), Marvel grass (Dichanthiumannulatum (Forks) Stapf), Alfalfa (Medicago sativa), Common read (Phragmites communisT.), Wormwood (Artemisia camperstis L.), Sesbania (Sesbania sesban), Purslane (Portulacaoleracea), Cucumber (Cucumis sativus) and Red bottle brush (Melaleuca viminalis), in Al-Dewania and Al-Najaf Al-Ashraf province field by using polymerase chain reaction (PCR) and find out the relationship between the phytoplasma that infect these plants with sesame phytoplasma. Phytoplasma identification was confirmed by PCR according to amplifying a 558 bp - DNA clearly band representing the samples of sesame(Sesamumindicum L.). All the plants gave positive results by giving 558 bp – DNA according to PCR test with the exception of Darnel (Lolium temulentum L.), Cynanchum (Cynan chumacutum L.) and worm wood weed plant (Artemisia camperstis L.) gave negative results. Sesame seeds collected from control and treatment field experiment gave positive result according to PCR test.
Keywords
Phyllody Phytoplasma; Sesame; PCR; Host range

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