Effect of Sperm Activation and Addition of Treated Sheep Follicular Fluid on the Quality of Awassi Cryopreserved Semen

Abdullah Zuairi Al- Sadoon, Ali; Kassar Jaser AlSaab, Hazim; Awad Gatea, Eqbal

	Effect of Sperm Activation and Addition of Treated Sheep Follicular Fluid on the Quality of Awassi Cryopreserved Semen
Kufa Journal for Agricultural Science
Article 1, Volume 8, Issue 4, 2016, Pages 184-192
Authors
Ali Abdullah Zuairi Al- Sadoon; Hazim Kassar Jaser AlSaab; Eqbal Awad Gatea
Abstract
This study was conducted at the laboratory of Higher study /Animal Resources Department /College of Agriculture /University of Al-Muthanna at the summer of 2015, to evaluate the effect of follicle fluid to improvement of fertilization ability (representing on individual motility and progressive motility) of ram sperms after cryopreservation- thawing . Twenty five cryo-semen straw for four Awassi rams were thawed gradually and evaluated initially for individual motility and progressive motility, than the semen was divided into two major part, part one was divided into four eppendrof tubes and extended with SMART medium treated with follicular fluid (FF) in concentrations with (0, 5, 10, 15%) , semen from this four treatment was evaluated for individual motility and progressive motility to understand the effect of FF on this characteristics'. Part two also divided into four eppendrof tubes and subjected into sperm activation using swim-up technique and extending with SMART medium treated with follicular fluid (FF) in concentrations with (0, 5,10,15%) and incubated under 37C˚ .After 45 minutes ,the four treatment was evaluated for individual motility and progressive motility to learning the effect of sperm activation with FF on improvement this characteristics as a model for semen quality . The results indicated that the activation was significantly (P<0.05) improve the individual motility(11.67±0.09) after activation as compared with (5.00±0.03) before activation , while the activation was highly significantly (P<0.01) improve the individual motility (30.00±0.27 and 41.67±0.32) and progressive motility (13.33±0.07 and 31.67± 0.23) of ram sperms with highest concentration (10,15%) of FF. According the result of the present study , there was an ability to improve the quality of Awassi ram semen after cryopreservation –thawing by applying the sperm activation (swim-up technique) with SMART medium treated with (10,15%) of FF before Artificial Insemination.
Keywords
follicular fluid; Semen; Awassi ram

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