BIO: DEGRADATION AND INHIBITION OF AFLATOXIN B1 PRODUCTION IN CORN GRAINS

H. Hussien, A.; N. Matny, O.

	BIO: DEGRADATION AND INHIBITION OF AFLATOXIN B1 PRODUCTION IN CORN GRAINS
IRAQ JOURNAL OF AGRICULTURE
Article 1, Volume 21, Issue 1, 2016, Pages 0-0
Authors
A. H. Hussien; O. N. Matny
Abstract
The study was conducted to evaluate the efficiency of Lactobacillus rhamnosus and Boswellia volatile oil to inhibit growth of toxigenic isolate of Aspergillus flavus and reduce aflatoxin B1production and its detoxification. The results showed that addition the bacteria at 2,3,5 ml/ L from bacterial culture containing 16 x 1021 CFU/ ml and the volatile oil at 6, 7, 8 ml/L into the yeast extract sucrose liquid medium (YSE) , then inoculated with A. flavus induced significant reduction in aflatoxin production, 73.7, 73.3, 53.3 g/L for the three tested concentrations of bacteria respectively, 130, 120, 90 g/L for the concentration of volatile oil respectively compared with 256.7 g/L in control. The addition of the bacterium at 2, 3, 5 ml/L and volatile oil at 7, 8, ml/L into liquid medium after 21days of inoculation with A. flavus and incubation at 25 + 2oC induced significant reduction in aflatoxin concentration, 150, 140,106.7 g/L for the three tested concentrations of bacterium respectively, 160, 146.7g/L for the concentrations, 7 and 8 ml/L in control. Treated corn grains with bacteria at 30, 40, 50 ml/500 g and the volatile oil at 8 ml/kg, then inoculated the grains with A. flavus, induced significant reduction in aflatoxin production, 70, 73, 74 g/kg for the three concentrations of bacterium respectively, 147.0 g/kg for volatile oil at 8 ml / kg compared with 267.0 g/ kg in control after 30 days of treatment. The treatment of corn grains with the bacterium at 30, 40, 50 ml/500 g and with Boswellia volatile oil at 8 ml/kg after 21 days of inoculation with A. flavus and incubation at 25 ± 2oC induced significant reduction in aflatoxin concentrations, 80, 47, 28 g/kg for the three concentrations of the bacterium respectively, 150 g/ kg for the volatile oil at 8 ml/kg compared with 267.0 g/kg in control.

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