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Comparative Study of Prepared Bromelain Gel Formulations and their Evaluation by HPLC Determination | ||
| Al-Mustansiriyah Journal for Pharmaceutical Sciences | ||
| Article 1, Volume 16, Issue 2, 2016, Pages 77-81 | ||
| Author | ||
| Kahtan Jassim Hasson | ||
| Abstract | ||
| Bromelain powder (proteolytic enzyme) was formulated as a gel for topical medical application by using two preparations method; Carbople 940 was used in the first formulation and Lutrol F 127 in the second. The physical and pharmaceutical properties of the gel formulations including the diffusion rates through the skin in vitro were evaluated. The best permeability of Bromelain was obtained with the 22% Lutrol F 127 gel formulation. In addition, a simple and rapid reversed-phase HPLC method was developed to monitor the quantitative analysis during the study. The used column was C18, 5µm (25 cm length), the mobile phase consisted of 70% methanol in 0.1 M dibasic potassium phosphate. The retention time of Bromelain was 7.3 minutes and the method proved precision as the straight line relationship of peaks areas and concentrations was with a correlation coefficient 0.998, and the RSD value for five successive determinations for same sample solution was 1.2%. | ||
| Keywords | ||
| Bromelain Gel; HPLC Determination; HPLC | ||
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