Molecular identification and characterization and phylogenetic study of six Eimeria species in cattle in Al-Najaf province

Jawad Ameer Al-Jubory, Qasim; Mohammed Ali Al-Rubaie, Haidar

	Molecular identification and characterization and phylogenetic study of six Eimeria species in cattle in Al-Najaf province
Euphrates Journal of Agriculture Science
Article 1, Volume 8, Issue 3, 2016, Pages 83-96
Authors
Qasim Jawad Ameer Al-Jubory; Haidar Mohammed Ali Al-Rubaie
Abstract
The study extended from April 2015 through January 2016 and included 600 cattle with varying age (1 day to 8 years). It was conducted in Al.Najaf province. Direct and flotation methods were used for the diagnosis of Eimeria infection. PCR and phylogentic tree was done for all isolates. Eimeria infection was reported in 211 out of 600 (35.2 %). PCR permitted the isolation of six Eimeria species. Nucleotide sequence was done and phylogenetic tree was established. The percentage of each species was as follows: E. zuernii(53.1%), E. cylindrical (49.3%), E. ellipsoidalis (34.6%), E. bovis(28.0%), E. auburnensis(22.7%) and E. alabamensis(19.4%).The Eimeriabovis, Eimeriaauburnensis, Eimeriacylindrica, and Eimeriazuernii were closely related together. The Eimeriaellipsoidalis appeared less related to other Eimeria species. Whereas, the Eimeriaalabamensis appeared significantly different from other Eimeria species and out off tree.For our knowledge this is the first molecular study done in Iraq for identification and characterization of Eimeria species in cattle. Molecular method can be utilized as a powerful, sensitive tool for the diagnosis of Eimeria infection in cattle, and as a superior substitute to the conventional morphologic methods. Phylogenetic tree analysis of the common six Eimeria species has been disclosed in this study to add to the original Japanese study by Kawahra in 2010.
Keywords
Phylogenetic tree; PCR; Eimeria

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