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Effect of Source of Explants and TDZ, IBA on Regeneration Plantlets of Gardenia jasminoides Ellis. cv. Radicans via Tissue Culture | ||
| Basrah Journal of Agricultural Sciences | ||
| Article 23, Volume 29, Issue 1, 2016, Pages 239-256 PDF (0 K) | ||
| DOI: 10.33762/bagrs.2016.116226 | ||
| Authors | ||
| Huda A. Al-Taha; Lamiaa H. M. Al-Mazine | ||
| Abstract | ||
| This study was conducted in Plant Tissue Culture Laboratory, Agriculture Collage, Basrah University, Basrah Governorate, Iraq during the period 23/ October/ 2013 to 23/ May / 2014. The aims of this study can be summarized to effect the source of explants and different concentrations of Cytokinins (TDZ), Auxins (IBA) on initiation, multiplication and rooting explants in micropropagation of dwarf gardenia (Gardenia jasminoides Ellis.) cultivar 'Radicans', Maximum successful response of shoot tips in vitro (100%) was noticed in MS medium enriched with different concentrations of TDZ (0.5, 1, 1.5 and 2 mg.L) in the presence of NAA and GA3 0.2mg.L each, but the high rate of shoot number(1.8) and length (1.18 cm) obtained on MS medium containing 1 mg.L TDZ. Where, the rate value of leaf width and numbers were increased only on MS medium fortified with low concentrations of TDZ (0.5 and 1mg.L). On the contrary, nodal explants cultured on same medium failed to responding, except the ones cultured on MS medium containing 1mg.L TDZ , showed moderate rate value of average growth responding(66. 06%). Highest shoot and leaf numbers and leaf width recorded on MS medium containing 1 mg. L TDZ in the presence of NAA and GA3 0.2mg.L each (4.33 shoots. explant, 6.00 leaves. explant and 1.30 cm respectively). Whereas, highest shoot length (1.50 cm) recorded on MS medium fortified with 0.5 mg. L TDZ. However, small amount of white brownish callus raised around the nodes grown on medium containing 0.5 mg. L TDZ, while big amount of the same callus was recognized around the nodes grown on MS medium containing 1.5 mg. L TDZ. Maximum root formation rate (100%) with high number of roots (7.33/plantlet), root length (7.33cm) and number of secondary roots (8.00. main root), were obtained within a short period (13.33 days) when the multiplied shoots transferred to½ MS medium enriched with 4mg.L IBA compared with another treatments (0, 1, 2, 3, 4) mg. L. Also, it was found that the length of shoots and number of leaves were increased to (12 cm and 11.33 leaves. plantlet, respectively). | ||
| Keywords | ||
| Plant tissue culture; Gardenia jasminoides Ellis; Radicans; TDZ; IBA | ||
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