Detection the genotyping of Cryptosporidium parvum isolated from human and calves and studying in vivo effecte of Sacharomyces boulardii on parasite

Taher Mohammed, Sabaa

	Detection the genotyping of Cryptosporidium parvum isolated from human and calves and studying in vivo effecte of Sacharomyces boulardii on parasite
Al-Mustansiriyah Journal of Science
Article 1, Volume 27, Issue 3, 2016, Pages 6-12
Author
Sabaa Taher Mohammed
Abstract
This study include the collection of 320 stool sample, (250) stool sample, were collected from patients of both sexes at different ages and seventy samples were collected from calves suffering from severe diarrhea, all samples Staining with Modified Cold Ziel-Neelson stain . The oocysts were used to extraction the DNA and detection of C. parvium DNA from human and calves by using two types of primers have been used for the detection of C. parvium parasite were (nest rRna 830bp and rRna 1.325bp Primer). In this study the rRna Primer (1.325bp) did not given any results for human and calves stool samples but nest rRna 830bp Primer give positive results band human sample exactly the same band of animal sample. This shows that the genus, which infected the human himself, which infect the animal .Also the experimental study showed that the orally inoculated S. boulardii to infected mice led to reducing the shedding of this parasite in feces of the mice since the first day and became (zero) in day (9th) post inoculation, compared with Spiramycin reach to zero at day (10 th), While control group persisted on shedding the parasite in feces with continuous up to day (10th) . The efficacy of the S.boulardii was (87.45%), While the Spiramycin efficacy was (85.11%). The histo pathological study of the mice intestines shown that the parasite causes mild atrophy of the mucosa layer and atrophy villi intestine with infiltration of inflammatory cells, while S.boulardii yeast showed high effective In repairing and restoring to intestinal tissue to the normal structure with the slight in creasing in numbers of goblet cells, compared with Spiramycin which led to an excessive increase in division of the goblet cells and cells mucosa layer.

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