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Optimum Conditions Of 5-Nucleotidase Activity In SeraOf Kala¬Azaric Patients. | ||
| Journal of the Faculty of Medicine | ||
| Article 1, Volume 48, Issue 3, 2006, Pages 331-334 | ||
| Author | ||
| Eaman A.S. AL-Rubaee | ||
| Abstract | ||
| Background: This research focuses on the characterization studies of 5v-Nucleotidase (5'-NT) of kala-azar patients' sera. 5V NT is one of the enzymes used as indicator of liver function test . In this study the optimum conditions for the activity of 5s - NT enzyme has been demonstrated for kala- azaric patients.Materials and methods: Thirty five blood samples were obtained from Baghdad Hospitals from untreated infants and children with kala-azar. 5'-NT activity was measured by following the method of Wood and Williams. Results: Optimum conditions of serum 5' - NT activity of kala- azaric patients obtained were 50 u L , 30 minutes ,0.8 mM,7.7 and 50'C) as (Volume of serum , incubation time , substrate concentration, pH and temperature) respectively | ||
| Keywords | ||
| Nuceotidase; serum and kala; azar | ||
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