Effect of Some Materials on Productivity and Activity of -Hemolysin which Produced by Methicillin Ressistant Staphylococcus Aureus

Jebur Khalaf, Khawlah; Farhan Ali, Zainab

	Effect of Some Materials on Productivity and Activity of -Hemolysin which Produced by Methicillin Ressistant Staphylococcus Aureus
Iraqi Journal of Science
Article 1, Volume 57, Issue 1, 2016, Pages 352-359
Authors
Khawlah Jebur Khalaf; Zainab Farhan Ali
Abstract
In this study 77 human isolates of Staphylococcus aureus were obtain from different clinical sources. The results showed that the number of isolates producing α-hemolysin was 32 isolates (41.55%) , while non- hemolysin producing was 45 isolates (58.45%) .The minimum inhibitory concentration (MIC) of methicillin which were 32 micrograms ml. The effect of CD-Cholesterol ,Cholesterol ,Cyclodextran(CD) ,Methicillin and Phosphate Buffer Saline(PBS) on α- hemolysin activity was study and the hemolytic Titer was:8 ،32.768 ,65.536 , 140.737.488.355.32 , 4.961408E + 25 respectively, while the effect of the same effect with Titer 67.108.864 and low Titer with PBS 3.96140812E + 25. The α – hemolysin toxin was partialy purified by ammonium sulfate precipitation. partial purified toxin was injected inside the cornea of rabbit's eye and the erosion of cornea formed with diameter: 8 mm after 8 hours of injection while the erosion of cornea which injected with CD-Cholesterol that mixed with partial purified toxin was 4 mm. CD-Cholesterol was used in treatment as a drops which added to the affected eye and led to a decrease in corneal erosion area from 8 mm to 4 mm.
Keywords
hemolysin; Methicillin; ressistant Staphylococcus aureus; Cholesterol; Cyclodextran; Methicillin and PBS

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