- All Journals
- Al-Qadisiyah University
- Basrah University
- Kirkuk University
- Mosul University
- The Iraqi Borad for Medical Specialization
- University of Anbar
- University of Technology
- Al Mustafa University
- Al-Zahraa University for Women
- Alnoor University
- Kunooz University
- Misan University
- Ninevah University
- Tikrit University
- University of Al-Hamdaniya
- University of Kerbala
Heavy Metals Tolerance and Antibiotics Susceptibilty Profiles of Staphylococcus aureus Strains Isolated From Clinical Sources in Baquba city | ||
| Diyala Journal For Pure Science | ||
| Article 1, Volume 13, Issue 1, 2017, Pages 130-144 | ||
| Author | ||
| Lina Abdulameer Salman Alsaadi | ||
| Abstract | ||
| Twenty- one Staphylococcus aureus isolated from various clinical samples (wound , ear , pus , urin and burn ) collected from Baquba General Hospital over a period ( April 2015 to June 2015 ).Out of 21 Staphylococcus aureus isolates , 17 (80.95%) were resistant to methicillin , while 4 (19.04%) were methicillin susceptible. Results of antimicrobial susceptibility showed high resistance to amoxicillin , amoxicillin/clavulanic acid , trimethoprime and cefotaxime with ratio 100% , 76.1% , 76.1% and 71.4% respectively, while isolates resistant oflaxacin with 4.7%. The results showed that 15 isolates of the total (21) of Staphylococcus aureus could produce β-lactamase with percentage (71.4%) , 5 isolates produce of Metalloβ-lactamase with (23.80% ) , and 4 isolates (19.04%) produce ESβLs. Staphylococcus aureus isolates have ability to tolerant the highest concentration of heavy metals like (Cobalt , Copper , iron , Mercury , Zinc) with ratio (57.1% , 52.3% , 66.6% , 33.3% , 61.9%) respectively. Additionally result of plasmid profile presented that all isolates of Staphylococcus aureus contained two band s of plasmid vary in size . Plasmid curing was carried out by acridin orang (256μg/ml) , cobalt and zinc resistance character was found to be present on the chromosomal DNA rather than the plasmid DNA whereas iron , copper and mercury resistance characters were found to be present on the plasmid . Curing result showed the loss of antibiotic and heavy metal resistance property for (cefotaxim , amoxicillin , erythromycin , ciprofloxacin , iron , copper and mercury ) from the isolates and confirms a relationship between antibiotic and heavy metal resistance with plasmid. | ||
| Keywords | ||
| Staphylococcus aureus; Heavy metals; Antibiotics; Curing; Plasmid | ||
|
Statistics Article View: 140 PDF Download: 25 |
||