Abstract
A study was carried out to isolate and identify microorganism associated with computer keyboards and mice. A
total of (250) samples were collected from two different objects (150) samples from the keyboards and (100)
samples from the mice. Samples were collected from different Computer centers places some computer
laboratories (multiple- user) in college of medicine, Al-Batol Teaching Hospital, Al-Jamhory Teaching Hospital
in Mosul City and Laptops of Single –user (staff rooms).The collected samples were inoculated on Nutrient
agar,, Salmonella –Shighella agar, Blood agar, Chogulate agar ,Maconkey agar ,Brucella agar and Minnitol Salt
agar .The isolates obtained were examined and identified by colonial morphology; biochemical characteristics
and Gram stain, 99.2% of total samples collected were contaminated with mixed bacterial growth. The bacteria
that have been isolates includes, Gram positive bacilli, Gram negative bacilli, Staphylococcus aureus ,
Staphylococcus epidermis ,Enterococcus, Escherichia coli, Streptococcus.The study indicated that the number of
microorganisms present on multiple-user computer keyboard and mice were greater than the single user
computer keyboards and mice. The presence of pathogenic and commercial bacteria on the computer keyboards
and mice indicates that they might acts as environmental vehicles for the transmission of potentially pathogenic
bacteria. Our data recommended that regular cleaning and disinfection of the different parts of computers must
be used to reduce the microbial load especially for multiple user work station |