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Purification of Arylesterase and study the Relationship with some biochemical parameters in type I Diabetic pateints | ||
| Tikrit Journal of Pure Science | ||
| Article 1, Volume 19, Issue 5, 2014, Pages 65-74 | ||
| Authors | ||
| Omar Y. Al-Abbasy; Nashwan I. Al-Lehebe; Shihab A. Al-Bajari | ||
| Abstract | ||
| Abstract: The research included determination of Arylesterase activity and its relationship with some biochemical parameters of Type I diabetic patients serum. Significantly decreasing (p<0.05) activities of antioxidant enzymes, namely, Arylesterase, Glutathion-S-transferase, Catalase and lipoprotein lipase activities, and non enzymatic antioxidants levels, Glutathione, vitamin C levels, as well as insulin hormone , while non significantly decreasing in Glutathione peroxidase activity, vitaminE and Ceruloplasmin were found in type 1 diabetic patients as compared to control. Our results were demonstrates a significantly increasing (p<0.05) in Lypoxygenase, Polyamine Oxidase, Superoxide Dismutase and Xanthine Oxidase activities, in addition to Glucose, Malondialdehyde, urea, creatinine, glycosylated hemoglobin and insulin resistance in patients compare to healthy subjects. There was a significant positive correlation between ARE and Glutathione peroxidase (r=0.421), Ceruloplasmin (r=0.525), glutathione (r=0.411, vitamins C(r=0.735) and E(r=0.611), while it was a significantly negative with Xanthine Oxidase (r= -0.597), glucose(r= -0.721), Malondialdehyde (r= -0.834) and HbA1c(r=- 0.843). The partial purification of Arylesterase from serum of Type I diabetic patients was studied by using dialysis, diethylaminoethyl cellulose ion exchange chromatography and electrophoresis techniques. One proteinous peak of ARE activity were obtained with specific activities of 0.944 Umg protein and with a molecular weight of 42666 dalton | ||
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