Study the effect for the use of pregnyl & clomiphincitrate Drugs & Green tea in several Physio logical, Biochemological and Histological in H 2 O 2 Induced Oxidative stress female albino rabbits

Abd AL-Muonaim Taha AL-Robiaee, Wasan; Saab- Ahmed, Muneef

	Study the effect for the use of pregnyl & clomiphincitrate Drugs & Green tea in several Physio logical, Biochemological and Histological in H 2 O 2 Induced Oxidative stress female albino rabbits
Tikrit Journal of Pure Science
Article 1, Volume 19, Issue 6, 2014, Pages 7-17
Authors
Wasan abd AL-Muonaim Taha AL-Robiaee; Muneef Saab- Ahmed
Abstract
Abstract This study was conducted at the Animal House of the General Company for the pharmaceutical industry in Samarra To know the effect of the drug clomiphene Street and pregnyl aqueous extract of green tea on ovary of Albino famale rabbits which have oxidative stress induced by hydrogen peroxide (0.5%)for (21) days, And knowledge of the impact of these transactions on some variables and blood biochemical and hormonal including: the packed cells volume(pcv), Blood Haemoglobin (Hb) , Erythrocyte Sedimentation Rate (ESR), White Blood Cells(WBC), And the concentration of each of the Cholesterol, TriGlyceride , High density lipoproteincholesterol HDL-C , Low density lipoprotein –cholesterol LDL-C, Very low density lipoprotein- cholesterol VLDL-C, Urea ،Uric acide, Creatinin, Luteinizing Hormone (LH), Follicles Stimulation Hormone (FSH), Prolactin Hormone, Esterogen Hormone, progesteron Hormone, Malondialdehyed ( MDA) , Glutathion , This study included a 30 rabbits (Sprauge Dawely) at age 12 to 16 months,and weight at(1400-1600kg) After laboratory testing and ensure safety of animal diseases were divided randomly into10 major groups, each group consist of 3 rabbits as follows;Control group., Hydrogen peroxide treated group (0.5%)., Pregnyl treated group (10 mg/kg body weight)., Clomephen citrate treated group (10 mg/kg body weight) .,Green tea treated group (150 mg/kg body weight) with Hydrogen peroxide ., Pregnyl treated group (10 mg/kg body weight) with Hydrogen peroxide., Clomephincitrate treated group (10 mg/kg body weight) with Hydrogen peroxide ., Pregnyl treated group (10 mg/kg body weight) with Green tea., Clomephincitrate treated group (10 mg/kg body weight) with Green tea ., Green tea treated group (150 mg/kg body weight), And after the end of the period of the experiment conducted some tests physiological and biochemical and histological showed influence follows 1 - The results showed that oxidative stress led to a significant decrease when the level of significance (p <0.05) compared with the control Intact in PCV، Hb، HDL-C ،Uric acid, Creatinin ،Esterogen, LH, Prolactin , FSH, GSH, While Increased ESR, WBCS، Blood Glucose، Cholesterol, TG، LDL-C, VLDL-C, Progesteron، MDA. 2- The results of this study that the treatment led to the events of Both drugs significant decrease at level of significance (p <0.05) compared with the control Intact (healthy)in PCV, Hb, Cholestero, lHDL-C, LDL-C, Uric acid، Creatinin، LH، FSH، Prolactin، GSH While Increased ESR, WBCS, Blood Glucose, TG، Creatinin، Progesteron، Esterogen، MDA. 3- Results of the study found that treatment of both drugs + H2O2 led to a significant decrease when the level of significance (p <0.05) compared with control infected PCV, Hb, ESR, Cholesterol, LDL-C, TG, VLDL-C, Urea, Creatinin, Prolactin, MDA While Increased Esterogen، LH، GSH . 4- The study results showed that treatment of abstract watery green tea led to a significant decrease (p <0.05) compared with the control healthy inﻲ ﻓ WBCS, Blood Glucose, Cholesterol, TG، VLDL-C, Urea, Uric acid, Esterogen, LH, FSH, MDA While Increased HDL-C، Creatinin، GSH . 5- The study results showed that treatment of both drugs + aqueous extract of Green tea to the occurrence of a significant decrease (p <0.05) compared with the control Intact (healthy)in WBCS ،Blood Glucose، Cholesterol, TG ، LDL-C، VLDL-C، Urea، Uric acid, MDA While Increased Creatinin, Esterogen, Prolactin . 6- The study revealed that the current real estate clomiphene Street and Pregnyl effect on histology of the ovary, as their treatment led to the emergence of the preparation of primary follicles in ovarian cortex and the back of a limited number of secondary follicles, Did not notice the presence of a vesicle Krav, while observed in the group of green tea that the surface of the ovary did not show the germ cells, while characterized by crust ovarian existence of a number of follicles developing container cells ovale and is surrounded by a number of follicular cells, it was noted in the group of hydrogen peroxide that crust ovarian characterized by the presence of hemorrhage inside the cavity of mature follicles also contained blood cells Alhmmerma some white blood cells, did not notice the presence of the egg inside the follicle, note that there is infiltration of inflammatory cells and Allmufah frequently around the perimeter of the follicle..

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