Detection of (aggR,SLT.I) virulence genes in clinical and environmental isolates of Escherichia coli

M .Raoof, D; Waa,; A. Mohammed, Saffana

	Detection of (aggR,SLT.I) virulence genes in clinical and environmental isolates of Escherichia coli
Tikrit Journal of Pure Science
Article 1, Volume 19, Issue 6, 2014, Pages 40-46
Authors
d M .Raoof; ; Saffana A. Mohammed
Abstract
Abstract A total of (134) samples were collected from inpatients and out patients attending Tikrit Teaching hospital from both sexes and different ages to determine E. coli genes (aggR, SLT.I).These specimens included 29 swabs from cases of Otitis media, 35 from Wound cases, 6 from Burn cases and 40 Urine samples from UTI cases and 24 diarrheal samples with respect to clinical samples, on the other hand the environmental samples (54) samples were collected from soil and water, of then 43 samples from soil and 11 water samples. Isolates were identified according to morphlogical ,microscopic and cultural characterstics .Identification results showed that 33 clinical isolates (24.6 %) were E. coli. distributed as: 3(10.3%) from Otitis media, 5(14.2%) from Wounds, 1(16.6%) from Burns, 10(25%) from UTI and 14(58.3%) from diarrheal cases, for environmental samples identification results showed that 11 environmental isolates (22.2%) were E. coli, distributed as 6 13.9% from soil and 6 54.5% water. Thirty isolates were chosen from different sites of infections (4 isolates from each infection sites) and from environmental isolates to evalute the prevalence of (SLT. I, aggR) genes in these isolates by PCR using specific primers.The results of PCR showed the presence of aggR gene in 8(100%) chosen isolates, and SLT. I gene is found in 4 (50%) isolates and then 2 (50%) from Wound and 2 (50%) from diarrheal cases. Function of these genes and through the results of the electrophoresis of polymerization product of the genes under study it can be concluded that the pathogenic strain Eenteroaggregative E. coli is the most common among environmental isolates and which were confirmed in terms of gene aggR which is a diagnostic gene for this pathogenic strain in Tikrit city environment, while the strain the most common among clinical isolates are pathogenic strain Enterohemorrhagic E. coli, which has been confirmed in terms of gene SLT.I which is a diagnostic gene for this pathogenic strain at the Tikrit Teaching Hospital.

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