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Detection of virulance genes PLcH , PLcN, exoS in Pseudomonas aeruginosa isolates of urinary tract infection | ||
| Tikrit Journal of Pure Science | ||
| Article 1, Volume 19, Issue 6, 2014, Pages 66-72 | ||
| Authors | ||
| Waad M. Raoof; Shameran M. Tawfiq | ||
| Abstract | ||
| Abstract: Collected 80 samples from patients with suffered of the urinary tract , cultured on different media and diagnosed isolates through phenotypic and culturing characteristics, showed the results of the diagnosis that the 20 isolates belonging to the type P. aeruginosa, tested the sensitivity of these isolates to antibiotics and the results showed resistance of all isolates to antibiotics Carbencillin,, Ceftriaxon, Cefixime by (100%) and demonstrated resistance by ( 60% ) of the Gentamicin Tobramicin, Amikacin and were less resistant to antibiotics Cefepenim, Norfloxacin, Imipenem in ratio(9.2%), ( 10.2% ) and ( 8% ) respectively , were selected 9 isolates depending on its ability to resist many of the antibiotics to evaluation the spread of genes PLcH, PLcN, exoS within these isolates by specialized primers using the technique of PCR, showed the results of PCR the presence of gene exoS which encoded external S enzyme in rate 9/7 isolates and by ( % 77.77 ) of the isolates and the gene PLcH which encoded external enzyme haemolysin (the blood haemolysis) in the 9/6 isolation at a rate (% 66.66 ) either gene PLcN encoded external haemolysin enzyme (the blood non haemolysis) in the 9/7 isolation at a rate (% 77.77 ) P. aeruginosa isolates of bacteria under study | ||
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