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Mechanism of mitotic block and inhibition of cell proliferation by alkaloids extraction from Convolvulus Scammonia at low concentrations | ||
| Diyala Journal For Pure Science | ||
| Article 1, Volume 8, Issue 3, 2012, Pages 464-471 | ||
| Authors | ||
| Ibrahim hade; Zenia T.A | ||
| Abstract | ||
| Alkaloids extraction from Convolvulus Scammonia inhibited CHO cell line (china hamster) proliferation by inducing a sustained mitotic block at the metaphase/ anaphase boundary. Half-maximal inhibition of cell proliferation occurred at 8 nM alkaloids, and mitosis was half-maximally block 8 nM alkaloids. Inhibition of mitosis was associated with formation of an incomplete metaphase plate of chromosomes and an altered arrangement of spindle microtubules that strongly resembled the abnormal organization that occurs with low concentrations of vinblastine and other antimitotic compounds. No increase in microtubule polymer mass occurred below 10 nM alkaloids. The results indicate that alkaloids shares a common antiproliferative mechanism with vinblastine. At its lowest effective concentrations, alkaloids appears to block mitosis by kinetically stabilizing spindle microtubules and not by changing the mass of polymerized microtubule | ||
| Keywords | ||
| microtubules; cell line; Immunofluroresence | ||
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