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Measurement of Zinc Concentration in Human Blood Serum Byspectophotometric Methods | ||
| Kerbala Journal of Medicine | ||
| Article 1, Volume 9, Issue 2, 2016, Pages 2491-2497 PDF (0 K) | ||
| Author | ||
| Hussain Hasan Kharnoob | ||
| Abstract | ||
| background: speciation of zinc means the determination of the concentration of chemical formula of zinc species which is more indicated than total concentration of zinc for clinical diagnosis. It was shown that atomic absortion spectrometry is good technique for measuring of total concentration of zinc after digestion of the serum with concentrated nitric acid and concentrated perchloricacid, while ionic concentration of zinc was determined by visible spectrometry. Study design and objective :this study was conducted control healthy people from the workers in Tikrit teaching hospital,gendar (10male + 10 female) and their ages are between (35-70(years. This study aimes to find relation between the concentration of bound zinc in human blood serum and ischemaic heart disease. Methods: bloods samples were collected from clincilay suspected patients who attend teaching hospital in salah-aldingovernate with sign and symtoms of ischemic heart disease (proven by specialist physician). Blood samples were centrifugated and serum was separated to examine for speciation of zinc concentration Results: the zinc was determined by two analytical methods. The first method is visible spectrophotometry, the absorbance was measured at wavelength 578 nm and the zinc label as ionic zinc. The second method is atomic absorption spectrophotometrymetry, the absorbance was measurd at wavelength 213 nm and the zinc label as total zinc. The bound zinc was mathmetical calculated, which is higher in patients than control groups Conclusion:according to the study data, it was concluded that the level of total zinc higher than ionic zinc in control and patient, and the levels of ionic and total zinc in patient groups are lower than their levels in control groups List of abbreviation: FAAS: Flame Atomic Absorption Spectrometry GFAAS: Graphite Furnace Atomic Absorption Spectrometry | ||
| Keywords | ||
| Spectrophotometer; Atomic; Bound; concentration; TOTAL; Ionic | ||
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