Micropropagation of Date Palm (Phoenix dactylifera L.)  Fursee cultivar  in vitro

J. Shareef, Hussein; M. W. AL-Mayahi, Ahmed; Q. Muhsin, Luna

	Micropropagation of Date Palm (Phoenix dactylifera L.) Fursee cultivar in vitro
Thi-Qar University Journal for Agricultural Researches
Article 1, Volume 5, Issue 1, 2016, Pages 472-484
Authors
Hussein J. Shareef; Ahmed M. W. AL-Mayahi; Luna Q. Muhsin
Abstract
The study was carried out during 2014 and 2015 at tissue culture lab. – Date Palm research center, Basrah university in order to micropropagation of rare Fursee cultivar using Shoot tip, Auxiliary buds, and primordial Leaf. The explants were placed on basal (MS) medium with activated charcoal and supplemented with different the concentrations of the auxin (NAA) Naphthalene acetic acid and the cytokinin (2ip) 2-Isopentenyl adenine at 3 mg l-1. The results showed that high average of primary callus proliferated using NAA at 30 mg l-1 and 2ip at 3mg l-1. The same treatment and Shoot tip explants also gave the highest response percentage of callus proliferated compared with control treatment and other explants. Use of the auxin NAA and the cytokinin (2ip) at 3mg l-1increased significantly the percentage of embryo callus and fresh weight of callus after two months of culture besides on control treatment. Also, the auxin (NAA) concentration of 10 mg l-11increased significantly the percentage of embryo callus and fresh weight of callus compared with other concentrations. However, use of the auxin (NAA) increased significantly proliferated of embryos somatic to all concentrations. Whereas the stage of first growth different significantly from the concentration. The concentration of 1 mg l-1 NAA decreased the period of first growth significantly compared with other concentration. Also, the concentration of 1 mg l-1 NAA increased germination percentage of embryos compared with the control treatment.

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