Genetic study on Providencia rettgeri isolate from different clinical cases in Al-Diwaniya city

Hussein, Dr.; N, A.

	Genetic study on Providencia rettgeri isolate from different clinical cases in Al-Diwaniya city
journal of al-qadisiyah for pure science(quarterly)
Article 1, Volume 15, Issue 4, 2015, Pages 1-11 PDF (0 K)
Authors
Dr. Hussein; A. N
Abstract
11 isolates were diagnosed belonging to Providencia rettgeri of 750 swab collected from intensive care units, burns lobbies, operation room and Urosurgery unit, also took swabs of medical devices and instruments used in hospitals in Al-Diwaniya city, for the period from 1/2/2012 to 3/1/2013. Isolates were diagnosed depending on molecular investigate to gene 16s Ribosomal. Electrophoresis on agarose gel showed that all isolates containig on plasmidial bands and a tow plasmidial pattern and a carrier of a gene blaTEM and don’t carry gene blaSHv and CTX. also all isolates containig aph gene encoded to resist Aminoglygocides antibiotics and non-container qnr gene encoded for resist Quinolone antibiotics. While all isolates contain tet39 gene carried on the plasmid that encod to resist tetracycline antibiotic. Also investigated some of the genes encoded for some virulence factor and it appeared that all isolates carrying the gene cap encoded to produce a capsule in addition to a gene pap encoded for the invasion of P. rettgeri on the host and its adhesion as well as all isolates contain the gene encoded for system sidrophore. The modes of transmission of microbial containing conjugations processes and transformation were studied and showed the possibility of plasmid transfer from donor cells the represented P. rettgeri to receptor cells the represented (E. coli MM294) And by 50% where it was noted that conjucation cells become resistant to many antibiotics in addition to transfer production recipe ß–lactamase. sodium dodsel sulfate (SDS) was used to make the process of curing to some isolates of P. rettgeri, this process was succeeded in getting rid of plasmidial bands belonging to the isolates as lost some of the characters of resistance to antibiotics and produce ß–lactamase.

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