Abdul Salam K.AL-Saffar, F., Razak K. Mahmood, A., I. AL- Barhawi, N. (2017). Investigation at the molecular level for a nifgenes to bacteria klebsiella pneumoniea the use of polymerase chain reaction (PCR). , 22(3), 58-62.
Fawz Abdul Salam K.AL-Saffar; Abdul Razak K. Mahmood; Najwa I. AL- Barhawi. "Investigation at the molecular level for a nifgenes to bacteria klebsiella pneumoniea the use of polymerase chain reaction (PCR)". , 22, 3, 2017, 58-62.
Abdul Salam K.AL-Saffar, F., Razak K. Mahmood, A., I. AL- Barhawi, N. (2017). 'Investigation at the molecular level for a nifgenes to bacteria klebsiella pneumoniea the use of polymerase chain reaction (PCR)', , 22(3), pp. 58-62.
Abdul Salam K.AL-Saffar, F., Razak K. Mahmood, A., I. AL- Barhawi, N. Investigation at the molecular level for a nifgenes to bacteria klebsiella pneumoniea the use of polymerase chain reaction (PCR). , 2017; 22(3): 58-62.

Investigation at the molecular level for a nifgenes to bacteria klebsiella pneumoniea the use of polymerase chain reaction (PCR)

Tikrit Journal of Pure Science
Article 1, Volume 22, Issue 3, 2017, Pages 58-62
Authors
Fawz Abdul Salam K.AL-Saffar; Abdul Razak K. Mahmood; Najwa I. AL- Barhawi
Abstract
This research was aimed at detection on three nitrogen fixation genes nifK , nifD , nifH in 21 K. pneumonia pathogenic isolates which obtained from Prof. Jean-Marc in Leicester British university by using PCR technique and four specific primers P1, P2 , P5 , P6 and compare it with K. pneumoniae 342 standard strain. The results showed presence of two bands for each isolate with M.W 0.5 Kbp comparing with 1 Kbp of the marker.
key words: non-symbiotic fix nitrogen, nif genes , Klebsiella pneumoniea 342
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