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Association of Growth Hormone Gene Polymorphism with some Production Traits in Iraqi Local Chicken | ||
| Journal of Tikrit University For Agriculture Sciences | ||
| Article 1, Volume 17, Issue 0, 2017, Pages 428-436 | ||
| Authors | ||
| M.M.J AlRekabi; Ismail habeeb ismail; A.A.H ALKhazraji | ||
| Abstract | ||
| This study was conducted in laboratories and poultry farm in the Ministry of Science and Technology/ Agricultural Research Directorate / Animal resources and fisheries center / poultry department during the period from 01/01 /2014 until 15/03/2015. The aim of This study was to investigate the associations of growth Hormone gene polymorphism in Iraq local chicken and some production traits. Blood samples collected from wing veins from 93 hens at 9 weeks of old. Genomic DNA were extracted and a fragment of 770 bp in size amplified using PCR-RFLP method. To determine the restriction site in intron 1 of GH gene of the chicken, MspI endonuclease was used and the resultant digested products were run on 1.5 % agarose gel studied. the fragment at GH loci with MspI restriction enzyme was revealed AA, AB and BB alleles. According to the results, recorded allele was: AA allele (529, 241 bp); AB allele (529, 373, 241, 156 bp) and for allele BB (373, 241,156 bp), respectively. The comparison of different genotypes indicated that polymorphism in the GH gene was significantly (P<0.05) associated with body weight at several age and stage at sexual maturity,and egg production for 100 days but there was no significant with the other traits(first egg weight ,age at sexual maturity . According to the results, GH genes could be candidate gene that can affect some body composition traits in Iraq local chicken therefore we can used Growth hormone gene polymorphism as genetic marker for selection program. | ||
| Keywords | ||
| Growth hormone; Gene; polymorphism; Local chicken | ||
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