Genetic Diversity Analysis for a Number of Broad Bean (Vicia faba)  Genotypes and Their Di Alleles Hybrids Using RAPD-PCR Markers

Z .Mukhlif Al-Sugmiany, Rafae; M. Azez. AL-Joburi, Jassim; H. Ali Al-Assie, Akeel

	Genetic Diversity Analysis for a Number of Broad Bean (Vicia faba) Genotypes and Their Di Alleles Hybrids Using RAPD-PCR Markers
Journal of Tikrit University For Agriculture Sciences
Article 1, Volume 17, Issue 0, 2017, Pages 579-595
Authors
Rafae Z .Mukhlif Al-Sugmiany; Jassim M. Azez. AL-Joburi; Akeel H. Ali Al-Assie
Abstract
In this study, eight Vicia faba genotypes from ICARDA as parents and their 28 half di alleles hybrids, carried in order to determine the DNA fingerprinting and genetic distance of these genotypes and hybrids. The concentration and purity of isolated DNA was in required rang. RAPD marker used in this study with 17 primers produced 132 loci, 119 loci of them were mix bands between parents & hybrids and 13 loci were main bands for all, which represent 90% and 10% respectively. Primers OPP-01, OPG-11and OPW-09 had the highest number of loci was 12, while the primer OPC-05 gave the lowest number of loci was only four. Those primers produced 2272 bands with all samples (parents and hybrids), (432) of them were main bands and 1840 were polymorphism bands represent 19% and 81% respectively. In general, the primers produced 10 unique bands and 8 were absent. Depending on the genetic distance, results show three different status of the hybrids, the first one has same distance between the parents, whilst the second hybrids were nearby to either parent and away from the others, as for the third hybrids case are move away of both parents genetically, and this shows that the hybrid has not inherit genetic material regularly from the both parents.
Keywords
fingerprinting; RAPD; PCR; hybrids; Vicia faba

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