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Bacteriological Characterization OF Some Gram Negative Bacteria Isolated From Vaginal Infections | ||
| Diyala Journal For Pure Science | ||
| Article 1, Volume 13, Issue 2, 2017, Pages 222-241 | ||
| Authors | ||
| Hadi R Rasheed AL-Taai; Abass A. Frhan; Zwida K. Khadur | ||
| Abstract | ||
| This study included collection 410 samples of vaginal swabs from patients woman's in hospitals of Baquba city for the period from 1/9/2015 to 31/12/2015.The results showed that the highest percentage of vaginitis in pregnant woman(65%) than in both nonpregnant and nonfruitl (30%,5%) respectively The results refer that (24%)Escherichia coli, (20%) Proteus mirabilis by using diagnostic phenotypic ,biochemical test and conifirm the diagnosis using VITEC 2 analyser The results of the investigation of some virulence factores showed the isolstes of Escherichia coli and Proteus mirabilis produce of haemolysin with percentage (50%) and(90%) respectively. The results showed that all isolates of Proteus mirabilis were produced urease by(100%),while the results detect that the isolates of Escherichia. coli is not able to produce this enzyme. The results showed that isolates of Proteus mirabilis produced protease by (45%), while Escherichia coli have not ability to produce this enzyme. Results showed that all isolates of Escherichia coli have ability to adhesion surface of epithelial cells of human by(100%),while the isolates Proteus mirabilis showed ability to adhesion by(85%).The minimum inhibitory concentration MIC for 6 antibiotics, that Ampicillin, Amoxicillin ,Amekacin ,Cefotaxime,Gentamycin ,Tetracycllin was determined .The MIC values of these antibiotics ranged between (512-1025≥),(32-1024≥),(4-64),(8-1024),(4-128),(4- 512)µml,respectively. The minimum inhibitory concentration MIC for disinfectant povidone iodine10%, chloroxyzylenol 3%(50,100,200,300,400,500)µ ml,respectively. Results Combination antibiotic Gentamycin and disinfectant povidone iodine10% reduced the ability of isolates for adherence.The results showed averiance as far their resistance to these antibiotics of Escherichia coli and Proteus mirabilis showed highest resistance rate 100% for Ampicillin and Amoxicillin. | ||
| Keywords | ||
| Bacterial Vaginosis; Virulence Factor; Grame negative bacteria | ||
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