KH. MAHMUOD, M., M. TURKEY, A., A. SULAIMAN, A. (2014). STUDY OF OPTIMUM CONDITIONS FOR PROTEASE ENZYME PRODUCTION BY PSEUDOMONAS AERUGINOSA ISOLATED FROM DIFFERENT CLINICAL SOURCES.. , 8(2), 23-30. doi: 10.37652/juaps.2014.123974
MOHAMMED KH. MAHMUOD; AHMED M. TURKEY; AHMED A. SULAIMAN. "STUDY OF OPTIMUM CONDITIONS FOR PROTEASE ENZYME PRODUCTION BY PSEUDOMONAS AERUGINOSA ISOLATED FROM DIFFERENT CLINICAL SOURCES.". , 8, 2, 2014, 23-30. doi: 10.37652/juaps.2014.123974
KH. MAHMUOD, M., M. TURKEY, A., A. SULAIMAN, A. (2014). 'STUDY OF OPTIMUM CONDITIONS FOR PROTEASE ENZYME PRODUCTION BY PSEUDOMONAS AERUGINOSA ISOLATED FROM DIFFERENT CLINICAL SOURCES.', , 8(2), pp. 23-30. doi: 10.37652/juaps.2014.123974
KH. MAHMUOD, M., M. TURKEY, A., A. SULAIMAN, A. STUDY OF OPTIMUM CONDITIONS FOR PROTEASE ENZYME PRODUCTION BY PSEUDOMONAS AERUGINOSA ISOLATED FROM DIFFERENT CLINICAL SOURCES.. , 2014; 8(2): 23-30. doi: 10.37652/juaps.2014.123974

STUDY OF OPTIMUM CONDITIONS FOR PROTEASE ENZYME PRODUCTION BY PSEUDOMONAS AERUGINOSA ISOLATED FROM DIFFERENT CLINICAL SOURCES.

Journal of University of Anbar for Pure Science
Article 9, Volume 8, Issue 2, 2014, Pages 23-30    PDF (0 K)
Document Type: Research Paper
DOI: 10.37652/juaps.2014.123974
Authors
MOHAMMED KH. MAHMUOD1; AHMED M. TURKEY2; AHMED A. SULAIMAN3
1Anbar Health Department - Public Health Department
2College of Science, University of Anbar
3University of Anbar - Center for Desert Studies
Abstract
Fifteen isolates of Pseudomonas aeruginosa isolated from different clinical sources ( 4 from ear, 3 from wounds, one from sputum, 3 from urine, 3 from burns, and one isolates from cerebral spinal fluid), these isolates were tested to know its efficiency to produce protease in liquid and solid medium in primary and secondary screening stages. Isolate marked as P5 obtained from burns infection was more efficient one in protease production. The optimum(temperature, pH,incubation period, and culture medium) for enzyme production were determined and the result showed that pH 8 , temperature 35 °C , incubation period 36 hrs were optimal conditions and tryptic soy broth medium for protease production.
Keywords
Pseudomonas aeruginosa
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