GENETIC DIVERSITY ANALYSIS FOR NUMBER OF DATE PALM VARIETIES IN AL-ANBAR USING THE RAPD-PCR TECHNIQUE

JAMAL HASHIM, KHALID; MAJEED A. HUMADI, ABDUL

doi:10.37652/juaps.2014.123975

	GENETIC DIVERSITY ANALYSIS FOR NUMBER OF DATE PALM VARIETIES IN AL-ANBAR USING THE RAPD-PCR TECHNIQUE
Journal of University of Anbar for Pure Science
Article 11, Volume 8, Issue 2, 2014, Pages 31-39 PDF (0 K)
Document Type: Research Paper
DOI: 10.37652/juaps.2014.123975
Authors
KHALID JAMAL HASHIM; ABDUL MAJEED A. HUMADI
College ducation university of Al-anbar
Abstract
The present study deals with the application of RAPD markers to study ten varieties of date palm in order to determine the genetic relationship and genetic distance among them. also aimed to determine the DNA fingerprinting for some of them. DNA was isolated from fresh leaves of date palm trees using CTAB method. The purity of DNA is ( 1.6-1.85 ) RAPD reactions had been conducted by using 12 primers. Three of them had not been detected, i.e. link sites which include OPN-16, OPZ-7 and OPF-10. Other nine primers show 617 bands 200 of them are main bands and 417 are a variety of Polymorphic bands. The primer OPW 13 has recorded the highest number of binding sites that makes 89 bands. The lower number of binding sites noticed 48 bands of the primer OPN 14. The ( Jouzi) cultivar has recorded the highest number of binding sites making 7 bands. The variety (Barban), however, is characterized with having the least number of binding sites by 40 bands.The results of genetic distance of the RAPD interactions had revealed that the minimum value of the genetic distance (0.0966) are between the two cultivar ( Ashrasee 8) and ( Dakwani 9), the highest value of genetic distance is (0.3214) between the two varieties ( Uwainat Ayyub 6) and ( Braban10). According to these values and regarding to genetic relationship , ten varieties had been classified into three main groups, the first group had included the variety( Ashrasi 8), ( Dakwani 9) and (Braban 10). The second group includes the two varieties ( Um Balaleez 2) and ( Asabia Alaroos 5). The third group includes two single varieties that include ( Assabia Alaroos 5) and ( Jouzi 4) and ( Maya 7). The second group includes the varieties ( Tibarzal, ( Khiarah 3) and( Uwaynat Ayyub 6)
Keywords
RAPD; PCR

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