Arrak Turkey, S., Fawzi Al-Ani, S., Jehad Al-hadithi, R. (2014). Comparison Between the Efficiency of nested PCR Analysis and IgG Avidity Test in Detection Of Acute Toxoplasma gondii Infection in Early Pregnancy in Ramadi city.. , 8(3), 1-6. doi: 10.37652/juaps.2014.123986
Saeed Arrak Turkey; Sarab Fawzi Al-Ani; Raja Jehad Al-hadithi. "Comparison Between the Efficiency of nested PCR Analysis and IgG Avidity Test in Detection Of Acute Toxoplasma gondii Infection in Early Pregnancy in Ramadi city.". , 8, 3, 2014, 1-6. doi: 10.37652/juaps.2014.123986
Arrak Turkey, S., Fawzi Al-Ani, S., Jehad Al-hadithi, R. (2014). 'Comparison Between the Efficiency of nested PCR Analysis and IgG Avidity Test in Detection Of Acute Toxoplasma gondii Infection in Early Pregnancy in Ramadi city.', , 8(3), pp. 1-6. doi: 10.37652/juaps.2014.123986
Arrak Turkey, S., Fawzi Al-Ani, S., Jehad Al-hadithi, R. Comparison Between the Efficiency of nested PCR Analysis and IgG Avidity Test in Detection Of Acute Toxoplasma gondii Infection in Early Pregnancy in Ramadi city.. , 2014; 8(3): 1-6. doi: 10.37652/juaps.2014.123986

Comparison Between the Efficiency of nested PCR Analysis and IgG Avidity Test in Detection Of Acute Toxoplasma gondii Infection in Early Pregnancy in Ramadi city.

Journal of University of Anbar for Pure Science
Article 1, Volume 8, Issue 3, 2014, Pages 1-6
Document Type: Research Paper
DOI: 10.37652/juaps.2014.123986
Authors
Saeed Arrak Turkey; Sarab Fawzi Al-Ani; Raja Jehad Al-hadithi
University of Anbar - College Of Medicine.
Abstract
Background: Primary maternal infection with toxoplasmosis during gestation and its transmission to the fetus continue to be the cause of tragic yet preventable disease in offspring. jective: This study was aimed to Comparison between efficiency of these two methods(nested PCR and IgG avidity test) in detection of acute toxoplasmosis in pregnant women early in the first trimester is of utmost importance in order to offer them early therapy or other interventions to prevent congenital infection of fetuses. Methods: One hundred sixteen blood samples were collected from pregnant women at different ages, and in the first trimester of pregnancy. These women have history of habitual abortion, intrauterine fetal death and congenital anomalies of the fetus. Blood samples were tested for specific anti-Toxoplasma IgG avidity test and detection of B1 gene of T. gondii by nPCR. Results: In this study, it was found out that the rate of Toxoplasmosis by nested PCR 48 cases (41.4%),while by T. IgG avidity test was 76cases(65.5%) (high avidity 54.3% and low avidity11.2%). The detection rate by nested PCR was significantly higher than by T. IgG avidity test. Conclusion: PCR technique is more sensitive and specific than T.IgG avidity tests.
Keywords
Toxoplasma gondii; Nested PCR; toxoplasmosis
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