- All Journals
- Al-Qadisiyah University
- Basrah University
- Kirkuk University
- Mosul University
- The Iraqi Borad for Medical Specialization
- University of Anbar
- University of Technology
- Al Mustafa University
- Al-Zahraa University for Women
- Alnoor University
- Kunooz University
- Misan University
- Ninevah University
- Tikrit University
- University of Al-Hamdaniya
- University of Kerbala
IN VITRO EVALUATION OF SOME NEW FUNGICIDES AGAINST SOME SOIL BORNE PATHOGENIC FUNGI | ||
| Journal of University of Anbar for Pure Science | ||
| Article 1, Volume 9, Issue 1, 2015, Pages 7-13 PDF (627.11 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.37652/juaps.2015.124288 | ||
| Author | ||
| BIHAR MOQDAD ABDULLAH | ||
| Abstract | ||
| Efficacy of three fungicides (Basten 50 SC , Rooty 72.2% SL and Ziram) were evaluated in vitro against three genera of plant pathogenic fungi : Alternaria sp. , Pythium sp. and Verticillium sp. by poisoned food teqnique at different concentrations i. e. (50, 100, 200, 300 and 400)% . Radial growth of mycelia was recorded after 3rd, 5th and 7th day of treatment. The fungicides efficiency was varied according to fungicide type, their concentration, fungi and incubation period. All the fungicides inhibited pathogenic fungi significantly as compared with controls. Basten fungicide had marked significant inhibitory effect against tested fungi as compared with Rooty and Ziram fungicides. Positive relationships between fungicide inhibitory and concentrations. Pythium sp. showed the highest sensitivity to fungicides represent by completely inhibited growth fungus (100% percent of inhibition), while Alternaria sp. was more resistant to fungicides, although presence of gradual decline in growth rate with increasing fungicides concentration to reach 18.33mm (67.14%) at 400% .Mycelial radial growth of Alternaria sp., Pythium sp. and Verticillium sp. proved differences in fungicides efficiency at different incubation periods (3rd , 5th and 7th days) which did not show a consistent pattern of growth under the effect of studied fungicides. | ||
| References | ||
|
1- Abou-Zeid A.M., Altahi A.D. and Abd El-Fattah R.I. 2008. Fungal control of pathogenic fungi isolated from some wild plants in Taif Governorate، Saudi Arabia. Mal.J.Microbiol. 4(1):30-39.
2- Kanwal A., Freeha A., Halima Q., Arshad J. and Rashid M. 2012. Evaluation of tebuconazole and thiophanate-methyl against some problematic soil-borne plant pathogens. Mycopath. 10(1):17-20.
3- Kakvan N., Hamidreza Z., Bahar M., Hossien T. and Shahab H. 2012. Study on pathogenic and genetic diversity of Alternaria alternata isolated from citrus hybrids of Iran, based on RAPD-PCR technique. European J. of experimental biology. 2(3):570-576.
4- Jeyaseelan E.C.,Tharmila S. and Niranjan K. 2012. Antagonistic activity of Trichoderma spp. and Bacillus spp. against Pyhtium aphanidermatum isolated from tomato damping off. Arch.Appl.Sci.Res.4(4):1623-1627.
5- Naraghi L., Amir A., Asghar H., Kasra S. and Homayoon A.A. 2013. Acomparison between Carbendazim fungicide and Talaromyes flavus controlling Verticillium wilt of potato under field conditions. International J. of Agricultural Science and Research. 4(1):89-100.
6- Johnson LE., Curl J., Bond, Fribourg, H. 1959. Methods for studying soil microflora-plant disease relationships. Minneapolis: Burgess Publishing Company.
7- Watanabe, Tsuneo. 2002. Pictorial atlas of soil and seed fungi : morphologies of cultured fungi and key to species. 2nd. ed.
8- Nene YL. and Thapliyal PL. 1993. Fungicides in plant disease control. Oxford and IBH Publishing Co. Pvt. Ltd., New Delhi.
9- Jalander V. and Gachande B.D. 2013. In-vitro efficacy of fungicides against the growth of leaf spot pathogen (Cordana musae (zimm) Hohn.) of Banana. Scince Research Reporter. 3(1):4-6.
10- Shinde V.N. and NasreJ. en S. 2011. Fungicidal efficacy on mycosis control of five leafy vegetable disease. Indian J. Sci. R. 2(3):53-58.
11- Milica M., Emil R., Javana H., Brankica T., I vana P., Milos S. and Svetlana M.M.2013. In vitro and In vivo toxicity of several fungicides and Timorex Gold biofungicide to Pythium aphanidermatum. Pestic. Phytomed.(Belgrade). 28(2):117-123.
12- Jamil S. and Manish K. 2010. Evaluation of fungicides against phyllosphere mycroflora of foliage plants. Biological Forum-An International Journal. 2(1):56-59.
13- Gomathinayagam S., Murugesan R. and Lydia O. 2013. Spot diagnosis of fungicide (Carbendazim) resistance in rice by using PCR with reference to Pyricularia oryzae. International Journal of Scince, Environment and Technology.2(5):1039-1058.
14- Ndikumana D. 2013.The effect of fungicide carbendazim on in vitro mycelial growth of two phytopathogenic fungi: case study of Fusarium oxysporum f.sp. lycopersici "strain F20" and Colletotrichum capsici "strain C226.3". East African Journal of Science and Technology. 2(2):76-96.
15- Delp C.J. and Klopping H. L.1968. Performance attributes of a new fungicide and mite ovicide candidate. Plant disease reporter.52:95-99.
16- Suleiman M.N.2011.The in vitro chemical control of Pythium aphanidermatum، an agent of tomato root rots in the north central, Nigeria. Scientia Africana.10(2):48-54.
17- Gorbach S. 1980. International union of pure and applied chemistry. A review of methods for the residue analysis of the systemic fungicides Benomyl, Carbendazim, Thiophanate methyl and Tiabendazole. Pure and Appl.Chem.52:2567-2590.
18- Yarden O., Katan J., Aharonson N. and Ben-Yaphet Y.1985. Delayed and enhanced degradation of Benomyl and Carbendazim in disinfested and fungicide-treated soils. Phytopathology.75:763-767.
19- Iqbal Z., Muhammad A., Salman A., Yasir I., Muhammad Y., Ali N., Usman M.,Altaf A. D. and Ahmed S.2010. Determination of minimum inhibitory concentrations of fungicides against fungus Fusarium mangiferae. Pak. J. Bot. 42(5):3525-3532.
20- Davidse L. C. and Flach W. 1977. Differential binding of methyl benzimidazole-2-YL carbamate to fungal tubulin as a mechanism of resistance to | ||
|
Statistics Article View: 2,537 PDF Download: 639 |
||