Comparative  diagnostic study of Klebsiella usingApi20E System and the device vitek2 and PCR

.Salah Salman Zain   AL-  Abdeen AL- Talabany, Dr; Mohammed Abd AL-Majed, Bnar

doi:10.32894/kujss.2017.124848

	Comparative diagnostic study of Klebsiella usingApi20E System and the device vitek2 and PCR
Kirkuk Journal of Science
Article 1, Volume 12, Issue 1, 2017, Pages 81-94 PDF (0 K)
DOI: 10.32894/kujss.2017.124848
Authors
Dr .Salah Salman Zain AL- Abdeen AL- Talabany; Bnar Mohammed Abd AL-Majed
Abstract
The current Study included 273 samples colected from models of different clinical Samples (urine , Stool, blood , sputum, and swabs from the vagina ,wounds, ear and throat) which have been get on 30 (10.98% ) isolates of Klebsiella from total of various clinical samples. The rate of Klebsiella from urine samples was 26.6% of the total 30 samples and The isolation rate from stool and blood sampls was 16.6%, while in wound infection the rat was (13.3%). Klebsiella isolated from vaginal swabs with (10%), and with respect to ear infections the percentage of isolation that have emerged in this study are (3.33%) and isolated rate from the throat and sputum was (6.6%). After a Diagnosis results have shown the prevalence of K.pneumoniae with rate(23 with76.66%) followed by K.oxytoca rate (5with16.66%) and 2 K.terrigena isolates with( 6.66%) of the total 30 isolates. Twenty isolates were selected belonging to the species K.pneumoniae and K.oxytoca which diagnosed by Api20E for rediagnosing by(PCR and viteke2) , and the results of the diagnostic system Vitek2 showd that the Klebsiella which have been previously diagnosed byApi20E system (90%) K.pneumoniae and (10%) K.oxytoca while the genetic diagnosis results showed 80% of the isolates belonged to the type of the K.pneumoniae and 20% of isolates belonging to K.oxytoca
Keywords
Klebsiella Vitek; PCR; Klebsiella; Vitek

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