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Developed use of silver nitrate in staining of bacteria | ||
| Kirkuk Journal of Science | ||
| Article 1, Volume 12, Issue 2, 2017, Pages 75-80 PDF (0 K) | ||
| DOI: 10.32894/kujss.2017.124935 | ||
| Authors | ||
| and Dr. ChnarNajmaddin; Dr.Fouad Hussein Kamel | ||
| Abstract | ||
| It has developed a method for the detection of bacteria using solution of Silver nitrate in concentration of 2%, particular to spore forming and capsulated types of bacteria. The bacteria were fixed on the slid, and adding prepared silver nitrate solution to stain bacteria cells such as (Mycobacterium tuberculosis, Bacillus sp. Klebsiella sp. bacteria formation capsule, and Staphylococcus aureus) on the slide and left for ten minutes at room temperature. Later, exposing the slid to ultraviolet rays for 25 minutes where the color change of fixed bacteria sample on the slide to red - light brown color as a result of the reduction of silver metal which penetrate the cell. As result brighten bacterial cells were examine under an optical microscope, which distinguishes the bacterial cells. | ||
| Keywords | ||
| Silver nitrate; Stain of bacteria; Silver nitrate for stain agarose; Silver nitrate staining principle; Silver nitrate staining protocol | ||
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