Optimization of  some  amylolytic enzymes (amylases) production by locally isolates of Bacillus spp

	Optimization of some amylolytic enzymes (amylases) production by locally isolates of Bacillus spp
journal of kerbala university
Article 1, Volume 13, Issue 1, 2017, Pages 55-67 PDF (0 K)
Abstract
Twenty Bacillus spp bacterial isolates (amylolytic enzyme producers) were obtained from 75 isolates which isolated from 12 soil samples from different parts of holly Karbala governorate , isolates number 5 and 17 were the most efficient for alpha-amylase and glucoamylase production respectively . Optimum environmental and culture condition has been determined for the production of alpha-amylase enzyme from isolate No. 5 by using the production media Soluble starch Yeast extract Trypton medium (SYT) that contains lactose with concentrate 1% as source of carbon and mixture of yeast extract and trypton with concentration 0.2% as source of nitrogen, the media wase support by NaCl2 , CaCl2 , MgSO4 , KH2PO4 and K2HPO4) with total concentration 0.26 as source of mineral salts . The primary pH adjusted to 7 , inoculum size was 5% and incubate for 24 hours in 42c̊ and 120 rpm/min. While the optimization of glucoamylase production from Bacillus licheniformis isolate no. 17 have been determined by using production medium (SYT) that contains soluble starch with concentration 1% as source of carbon and yeast extract with concentration 0.2 % as source of nitrogen. The media was supported by ( Nacl , CaCl2 , KH2PO4 and K2HPO4) with total concentration 0.26 as source of mineral salts. The The primary pH adjusted to 6.5 , inoculum size was 5% and incubate for 24 hours in 37c̊ and 120 rpm/min.

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