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Molecular detection of Coxiella burnetii in human and sheep in Al-Diwaniyah province by Real Time- PCR | ||
| Kufa Journal For Veterinary Medical Sciences | ||
| Article 1, Volume 7, Issue 2, 2016, Pages 193-203 | ||
| Authors | ||
| Khetam Qaid AL-Hamdawee; Noaman Naji Aaiz; Ghasoun Mohammed-Ali Wadai | ||
| Abstract | ||
| Background: Iraq is agricultural country, but the important aspect of livestock shown by most of the studies conducted in previous years, which dealt with Q fever infection in animals ( cattle, sheep and goat ) strikingly. Q fever it is a worldwide zoonotic disease caused by Coxiella burentii . Material and methods: One hundred and five (105), human samples used in the study were (50) (22 sputum/28 bronchial-alveolar lavage) collected from Teaching Hospital and Center Pulmonology and Respiratory Specialist in Al-Diwaniyah. Collected sheep samples were (55) Sample (28 nasopharyngeal swabs and 27 bronchial fluids) from the slaughterhouse of Al-Diwaniya during January – august 2016. All samples were collected from peoples & sheep that suffer from clear respiratory signs. By using Sybr Green Real Time- PCR technique the samples tested directly. Results: showed that the infection incidence in humans (16%) and in sheep (38.181%), the study proved that there were a significant differences at the site of infection in the respiratory tract (URT& LRT) for humans and sheep alike, as well as in terms of age groups and gender. Conclusions: Q fever is a serious disease circulating between peoples in Al-Diwaniyah city and clearly defined risk groups: people with respiratory infection, heart and vascular disease, immunosuppressed patients and distributed in sheep that have respiratory disorder. Real Time –PCR is a potential tool to diagnosis of C. burnetii in humans and animals, because of its rapid and sensitive detection. | ||
| Keywords | ||
| burnetii; human; Sheep; real time; pcr SberGreen; respiratory samples; Iraq | ||
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