Molecular detection  of Hydatid cyst isolated from Goats  in AL-Qadisiyia province by Polymerase Chain Reaction

Jadaan Ali Alkhaled, Mansoor

	Molecular detection of Hydatid cyst isolated from Goats in AL-Qadisiyia province by Polymerase Chain Reaction
Kufa Journal For Veterinary Medical Sciences
Article 1, Volume 6, Issue 1, 2015, Pages 191-195
Author
Mansoor Jadaan Ali Alkhaled
Abstract
The present study was conducted during the period from August 2013 to April 2014 in AL-Qadisiyia province to detect the infection of hydatidiosis in Goat using Molecular and conventional methods. One hundred and seventy nine Goat were examined at slaughters in Aldewanyia abattoir. Caprine hydatid cyst were removed from infected liver and lung after visceral inspection of these organs then investigated the viability of protoscolices by staining with eosin stain then examining under light microscope of 40x.The results showed 45 hydatid cyst were found from 179 goat , the prevalence rate of infection (25.13%) . 20 cyst were found fertile only whereas 25 cyst were sterile. Regarding to infection site in organs, out of 45 hydatid cyst collected , 28(62.22%) cyst were isolated from liver whereas 17 (37.77%) isolated from lung with significant differences at p<0.05. Out of 45 hydatid cyst samples were taken from infected goats, and examined by conventional PCR , the PCR product demonstrated an expected fragment of 440 bp in the length , 10samples were given positive cases , 7(25%) samples from liver whereas only 3(12%) from lung samples gave positive PCR result, with statistically significant difference (P<0.05) between two organs.
Keywords
Echinococcsus granulosus; Hydatidosis; Hydatid cyst; goat; PCR

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