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Molecular identification of Fusarium spp. isolated from tomato plant in Iraq and China | ||
| Basrah Journal of Agricultural Sciences | ||
| Article 10, Volume 30, Issue 1, 2017, Pages 65-72 PDF (0 K) | ||
| DOI: 10.33762/bagrs.2017.127317 | ||
| Authors | ||
| Iman S. Al-Jaafari; Adnan I. Al-Badran; Mohammed A. Fayyadh | ||
| Abstract | ||
| This study was conducted to identify Fusarium spp isolated from tomato plant in Iraq and China. 12 isolates from Iraq and four isolates from China were used in this study. Based on Morphological characteristics (color, growth pattern, Macro and Micro-conidia shape) high differences between Fusarium isolates were found. Sensitivity test to the fungicide carbendazim revealed that one third of isolates(4/12) from Iraq had EC50 values over than 1000µg/ml indicated that many Iraqi isolates have developed resistance to carbendazim. Based on ITS sequences, Fusarium isolates which were isolated from Iraq and China were identified as follow, isolates 1, 3, 5, 6, 7, 10 were identified as Fusarium oxysporum, isolates 8, 11 as F. solani, isolates 12, M1 and M3 as F. moniliforme, isolates 2, M2 and M4 as F. proliferatum, F. chlamydosporum and F. kyushuense respectively. Isolates from1-12 were isolated from Iraq while M1-M4 from China. | ||
| Keywords | ||
| RAPD; PCR; Fusarium spp; tomato plant; Iraq; China | ||
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