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Molecular Detection of virulence factor genes in pseudomonas aeruginosa isolated from human and animals in Diwaniya province | ||
| Kufa Journal For Veterinary Medical Sciences | ||
| Article 1, Volume 8, Issue 1, 2017, Pages 218-230 | ||
| Author | ||
| Azhar Abdulsada Neamah | ||
| Abstract | ||
| Virulent factors are molecules that produced by pathogenic Pseudomonas aeruginosa that enable it to play a great role in causes of the disease , these virulence factors encoding by virulence genes located in the chromosome of Pseudomonas aeruginosa. However, this study was examined four virulence factors genes included (toxA) that encoding to exotoxin A , (exoS) that encoding to exoenzyme S , outer membrane protein (oprL), and outer membrane lipoprotein I (oprI) that encoding surface protein (lipoprotein) by use PCR technique based using specific primers were design in this study.(50) swab samples were collected from human clinical infection samples from Al-Diwanyia hospital, and (30) milk samples were taken from cattle milk that infected by mastitis from different fields in Al-Diwanyia province. The samples were cultured on selective media and then extracted DNA followed by PCR technique to detect the virulence genes. The results were shown a clear difference in isolation of P. areuginosa isolates from human and animal source. Where, in human the percentage of P. areuginosa in wound was (42.8%),in the burn was (70%) , and Otitis media was (68.7%), while, in cattle the percentage of P.aeruginosa in milk was (26.6%); Where percentage of ToxA genes was (100%) in the wound and cow milk; and it was (92.8%) and (81.8%) in burn and Otitis media respectively. while , exoA gene were (66%) , (57.1%), (45.4%) and (75%) while were oprL gene was (50%) ,(50%) , (54.5%) and (50%) , oprI were (66%) ,(42.8%) , (54.5%) and (50%) in Wound , Burn, Otitis media , Cattle milk isolates respectively .In conclusion,use of PCR technique which given high specific and accurate results for detection of virulence factors genes in P.aeruginosa isolates that responsible for cause in disease in human and animals. | ||
| Keywords | ||
| pseudomonas aeruginosa; virulence factor genes; human and animals; PCR | ||
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