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Genetic identification for Staphylococcus aureus by using nuc gene | ||
| Journal of University of Anbar for Pure Science | ||
| Article 15, Volume 9, Issue 3, 2015, Pages 66-71 PDF (773.62 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.37652/juaps.2015.127590 | ||
| Authors | ||
| Mushtaq T. Salih* 1; Ahmed M. Turkey2; Mayada A. Shihan2 | ||
| 1University of Anbar - College of Pharmacy | ||
| 2University of Anbar - College of Science | ||
| Abstract | ||
| To examine the recovered strains phenotypically by conventional methods and genotypically by using synthetic oligonucleotides of 21 and 24 bases in polymerase chain reaction to amplify a sequence of nuc gene , which encoded thermostable nuclease of Staphylococcus aureus. A DNA fragment of approximately 270 bp was amplified from isolated DNA, PCR products was detected by agarose gel electrophoresis. Our results showed that 100% of Staphylococcus aureus were positive to nuc gene .The PCR for amplification of nuc gene has potential for rapid diagnosis of S. aureus infections by direct testing of clinical specimens . | ||
| Keywords | ||
| Nuc gene | ||
| References | ||
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