A. Abed, A., M. Turky, A., A. Abid, M. (2015). Extraction and Purification of Cellulase form Sphingomonas paucimobilis Isolated from deciduous trees leaves. , 9(3), 72-79. doi: 10.37652/juaps.2015.127591
Ali A. Abed; Ahmed M. Turky; Mohammed A. Abid. "Extraction and Purification of Cellulase form Sphingomonas paucimobilis Isolated from deciduous trees leaves". , 9, 3, 2015, 72-79. doi: 10.37652/juaps.2015.127591
A. Abed, A., M. Turky, A., A. Abid, M. (2015). 'Extraction and Purification of Cellulase form Sphingomonas paucimobilis Isolated from deciduous trees leaves', , 9(3), pp. 72-79. doi: 10.37652/juaps.2015.127591
A. Abed, A., M. Turky, A., A. Abid, M. Extraction and Purification of Cellulase form Sphingomonas paucimobilis Isolated from deciduous trees leaves. , 2015; 9(3): 72-79. doi: 10.37652/juaps.2015.127591

Extraction and Purification of Cellulase form Sphingomonas paucimobilis Isolated from deciduous trees leaves

Journal of University of Anbar for Pure Science
Article 16, Volume 9, Issue 3, 2015, Pages 72-79    PDF (696 K)
Document Type: Research Paper
DOI: 10.37652/juaps.2015.127591
Authors
Ali A. Abed* 1; Ahmed M. Turky2; Mohammed A. Abid2
1Anbar Education Directorate
2University of Anbar - College of Science
Abstract
Total of 15 bacterial isolates from cellulose degrading bacteria were obtained from deciduous trees leaves, these isolates varied in their ability to degrade cellulose, results of diagnosis of most efficient isolate was prove that it belong to Sphingomonas pucimobili. The results of enzyme purification produced from this bacteria using modified medium which extracted by Amicon Ultrafiltration showed that enzyme activity was 1.16 U/mg of protein and the saturation value with ammonium sulphate was 0-40% with enzyme accumulation 9.47% in total purification number of 2.46, in ion exchange chromatography the results of enzyme accumulation reached 11.92% and total purification number was 5.80 while the accumulation value was 8.63% and total purification number was 12.9 when gel filtration was used. The molecular weight of extracted and purified cellulase enzyme was 41 kDa calculated by poly acrylamide gel electrophoresis
Keywords
cellulase enzyme; Chromatography; SDS; PAGE; Sphingomonas pucimobili
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