M. Hamed, N., M. Turkey, A., J. Fahad, H., M. Al-Mahdawi, M. (2015). The relationship between genotype using BOX-PCR technique and lead bioremoval by Klebsiella pneumoniae. , 9(3), 80-89. doi: 10.37652/juaps.2015.127608
Nuhad M. Hamed; Ahmed M. Turkey; Harith J. Fahad; Mahmood M. Al-Mahdawi. "The relationship between genotype using BOX-PCR technique and lead bioremoval by Klebsiella pneumoniae". , 9, 3, 2015, 80-89. doi: 10.37652/juaps.2015.127608
M. Hamed, N., M. Turkey, A., J. Fahad, H., M. Al-Mahdawi, M. (2015). 'The relationship between genotype using BOX-PCR technique and lead bioremoval by Klebsiella pneumoniae', , 9(3), pp. 80-89. doi: 10.37652/juaps.2015.127608
M. Hamed, N., M. Turkey, A., J. Fahad, H., M. Al-Mahdawi, M. The relationship between genotype using BOX-PCR technique and lead bioremoval by Klebsiella pneumoniae. , 2015; 9(3): 80-89. doi: 10.37652/juaps.2015.127608

The relationship between genotype using BOX-PCR technique and lead bioremoval by Klebsiella pneumoniae

Journal of University of Anbar for Pure Science
Article 17, Volume 9, Issue 3, 2015, Pages 80-89    PDF (852.8 K)
Document Type: Research Paper
DOI: 10.37652/juaps.2015.127608
Authors
Nuhad M. Hamed* 1; Ahmed M. Turkey2; Harith J. Fahad3; Mahmood M. Al-Mahdawi2
1Anbar Education Directorate
2University of Anbar - College of Science
3University of Baghdad - College of Science
Abstract
The effect of metabolic status on lead bioremoval byKlebsiella pneumoniae were tested. The results revealed a significant differences (P<0.05) between live and dead (killed by heat) cellsas the bioremoval reached 57.0 ± 3.4 and 21.6 ± 2.5, respectively.Bioremoved lead ions were recovered (P<0.05) using three different washing solutions, 0.1M EDTA was the most efficient since it recovered about 63.9 ± 5.6 mg/ml, while Na2CO3 was able to recover 35.9 ± 2.6 mg/ml; whereas 0.1M HCl recovered 21.8 ± 2.6 mg/ml. genomic DNA was extracted from different isolates of K. pneumoniae in order to study the relationship between genotype using BOX-PCR and bioremoval of lead. Three clusters were detected C1, C2, and C3 with similarity percentages reached 52, 60, and 100%. What's more, bioremoval did not depend on particular genotype
Keywords
box; PCR; Klebsiella pneumoniae
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