Patterns of Phenotypic and Genotypic Resistance to Macrolides, Lincosamides and Streptogramins Group of Antibiotics by Efflux Pump and Enzymatic Modification in Methicillin Resistant Staphylococcus aureus

Saeed Mohammed, Luma; Talib Flayyih, May

	Patterns of Phenotypic and Genotypic Resistance to Macrolides, Lincosamides and Streptogramins Group of Antibiotics by Efflux Pump and Enzymatic Modification in Methicillin Resistant Staphylococcus aureus
Iraqi Journal of Science
Article 1, Volume 58, Issue 2, 2018, Pages 815-819
Authors
Luma Saeed Mohammed; May Talib Flayyih
Abstract
The study was carried out to isolate Staphylococcus aureus isolates from differents clinical samples to detect resistance of isolates to methicillin and detect the phenotypic and genotypic patterns of resistance to macrolides, lincosamides and streptogramins (MLS). The results showed that from 300 clinical sample there were 40 isolate related to S.aureus and there were 38(95)% of isolates were methicillin resistance S.aureus (MRSA). The suscepltibility test showed that there was 55% of isolates were resistance to erythromycin , 35% were resistance to clindamycin and 2.5% had intermediate resistance to the last one . The resistance to streptogramins determined phenotypicallyby the using of vitek 2 compact system,the results showed that there were four types of MLS resistance (iMLS, cMLS, M phenotype and SAB). All isolates were subjected to polymerase chain reaction teqnique in a monoplex pattern to amplify resistance encoding genes msrA, msrB,linA/linA’ and vga .The results showed that 36(90)% of isolates contain msrA(940bp) and msrB(595bp) , 30 (75)% of isolates contain linA/linA’ (323bp) gene while a region of (470bp) of vga gene was not found in any isolate.
Keywords
aureus; MRSA; iMLSB; cMLS; M phenotype; SAB; msrA; msrB; linA; vga

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