- All Journals
- Al-Qadisiyah University
- Basrah University
- Kirkuk University
- Mosul University
- The Iraqi Borad for Medical Specialization
- University of Anbar
- University of Technology
- Al Mustafa University
- Al-Zahraa University for Women
- Alnoor University
- Kunooz University
- Misan University
- Ninevah University
- Tikrit University
- University of Al-Hamdaniya
- University of Kerbala
Inhibition of Tomato Mosaic Virus replication in tomato plant Lycopersicon esculentum by Trichoderma harzianum mill fungi and its supernatant which filteration with chlorophorm. | ||
| Journal of University of Anbar for Pure Science | ||
| Article 24, Volume 9, Issue 3, 2015, Pages 136-143 PDF (581.25 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.37652/juaps.2015.127625 | ||
| Authors | ||
| Rajaa fadhil hamdi* ; Sajid Salahuddin Saleem | ||
| University of Anbar / College of Science | ||
| Abstract | ||
| The study was conducted to know the effect of crud filtrate of T. harzianum and its supernatant which screened with chlorophorm in replication of Tomato mosaic virus in tomato plant Lycopersicon esculentum and relied Datura stramonium plants as indicator plant to determind the number of local lesion, the results showed the positive effect of protein sediments in reduce the number of local lesion in Datura plants which which gave 3.145 lession/ cm2 while supernatant gave 5.236 lession/ cm2 and phosphate buffer treatment gave 7.176 lession/ cm2 .The results showed that the treatment of sidements proteins from supernatant which filtrate with chlorophorm reduced the number of local lesion with rate 5.295 lession/ cm2 compared with two control treatment ( water, potato media) which gave 8.212, 8.333 lession/ cm2 respectivly, while the crud filtrate treatment gave rate 5.012 lession/ cm2 , that mean, the treatment of crud filtrate succese to reduce the number of lesion and reduced the virus replication comparison with protein extract. Also, boiling the crud filtrate and protein extract led to losing the activity of both treatments, because the protein substances lost there inhibition activity when they exposed to high heat degree.The concentration 10-1 for crud filtrate and its filtrate screening with chlorophorm gave the grate effect in inhibition the virus in cm2 then the concentration 10-2 then 10-3 . | ||
| Keywords | ||
| Trichoderma harzianum | ||
| References | ||
|
1 - شریف، جنور هادی. 2004. استجابة بعض اصناف الطماطة للمسافات الزراعیة ومستویات النایتروجین فی منطقة السلیمانیة. رسالة ماجستیر، کلیة الزراعة، الجامعة المستنصریة. 2- الاسود، ماجد بشیر، عمر فوزی و امجد بویا. 2000. مبادیء الصناعات الغذائیة. مطبعة جامعة الموصل ص 22. 3- FAO. 2008. List of countries by tomato production.
4 - مدیریة الاحصاء الزراعی، الجهاز المرکزی للاحصاء. وزارة التخطیط. 2012. انتاج المحاصیل والخضراوات لسنة 2011. ص 10. 5- Duart, K.R.; H.luiz and C. A. Flavio.2001. Monoclonal antibodies to identify tomato mosaic tobamovirus. Braz. J. microbial. 32: 240 – 242.
6 - الجیرو، اناهید وعد الله. 2010. دراسات تشخیصیة على بعض فایروسات الطماطة فی محافظة نینوى، اطروحة دکتوراه، کلیة الزراعة والغابات، جامعة الموصل. 7- Gulser. D, Yllmaz NK, Candemir, F. 2008. Accumulation of Tobacco mosaic virus (TMV) at different depths clay and loamy sand textural soils due to tobacco waste Application. Environ. monit. Assess 146: 235 - 242.
8- Hull, J. 2002. Matthews plant virology. Academic press 1001 pp.
9- شوکت، عبد اللطیف بهجت .1982. فایروسات النبات، خصائصها، الامراض التی تسببها، مقاومتها. مطابع مدیریة دار الکتب للطباعة والنشر. جامعة الموصل. 10- Cook, RS. 2000. Advances in plant health management in the 20th century. Annu. Rev. phytopathol. 38: 95 – 116.
11- Megahed, A. A.; Kh. A. El - Dougdoug; B. A. Othman; S. M. Lashin; M. A. Ibrahim and A. R. Sofy. 2013. Induction of resistance in tomato plants against Tomato mosaic tobamovirus using beneficial microbial isolates. Pakistan Journal of biological sciences, 16 (8): 385 – 390.
12- Zaitlin. M. and H.W. Israel. 1975. Tobacco mosaic virus type strain C.M.I. / A.A.B. Description of plant viruses, No. 151.
13- Chambers, S.M. and E. S. Scott. 1995. In vitro antagonism of phytophthora cinnamomi and P. citricola by isolates of Trichoderma sp.and Gliocladium virens. J. phytopathol 143: 471 – 477.
14- السعیدی، ساجد صلاح الدین سلیم. 2004. تحفیز المقاومة فی نباتات الطماطة Lycopersicon esculentum Mill ضد الاصابة بفایروس موزائیک الطماطة Tomato Mosaic Virus عن طریق مستخلصات نباتیة. اطروحة دکتوراه، کلیة العلوم، الجامعة المستنصریة. 15- فلیجة، احمد نجم الدین وجمیل نجیب عبد الله. 1987. علم الخرائط والدراسة المیدانیة. الطبعة الثانیة. مطبعة العانی، بغداد. 16- قاشا، فاضل یوسف عبو. 1988. تحفیز المقاومة المکتسبة ضد فایروس موزائیک التبغ فی اوراق التبغ غیر الملقحة بالفایروس. رسالة ماجستیر، کلیة العلوم، جامعة صلاح الدین. 17- Wilson, E.G. 1998. Medical plant analysis. Academic press, New York. Vol. 2, p. 192.
18- Simons, N. John., R, Swidler., and L.M. Moss. 1963. Succulent type plants as sources of plant virus inhibitors. Phytopathology, 35: 677 – 683.
19- Chen YF, Zhau Y, Zhao XM, Guo P, An HL, Du YG. Han YR, Liu H and Zhang YH. 2009. Functions of oligochitosan induced protein kinase in Tobacco mosaic virus resistance and pathogenesis related proteins in Tobacco. Plant physiol bioch 47: 724 – 731.
20- Yan luo, D.D. Zhang, X.W. Dong, PB. Zhao, L.L. Chen, XY. Song, X.J. Wang, X.L. Chen, Mei, S. and YZ., Zhang. 2010. Antimicrobial peptaibols induce defence responses and systemic resistance in tobacco against tobacco mosaic virus. Lab of microbial Technology, marine biotechnology research center, Shandong university. FEMS microbial let 313. P 120 – 126.
21- Sticher, L.; Mauch – mani, B. and metraux, JP. 1997. Systemic acquired resistance. Ann Rev phytopathol 35: 235 – 270. | ||
|
Statistics Article View: 505 PDF Download: 430 |
||