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Molecular Variation Study of Clinical Isolates of Pseudomonas aeruginosa for 16SrRNA,pvdE, toxA, and phzM Genes Related with Virulence and Characterization Features. | ||
| Journal of University of Anbar for Pure Science | ||
| Article 25, Volume 9, Issue 3, 2015, Pages 144-155 PDF (1005.9 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.37652/juaps.2015.127638 | ||
| Authors | ||
| Ahmed M. Yuosif* 1; Ahmed M. Turky2; Ahmed A. Sulaiman3 | ||
| 1Ministry of Health - Anbar Health Department | ||
| 2University of Anbar - College of Science | ||
| 3University of Anbar/ Center for Desert Studies | ||
| Abstract | ||
| This study includes collection of 438 clinical samples from Ramadi Educational Hospital and 50 of soil samples in period from Nov. 2014 to Feb. 2015, to isolate Pseudomonas aeruginosa and the resulted isolates were divided into 40 clinical isolates form burns, wounds , urine and ear inflammation, and 10 from soil.Antiobiotic sensitivity test were done against 12 antibiotic discs for all 50 selected isolates by disc diffusion method, and the results indicated that all clinical isolates were resistance to three types of antibiotics(Penicillin, Ampicillin, Amoxicilline) while they varied in their resistance to other antibiotics. The soil isolates were 100% sensitive to all antibiotics except for penicillin and ampicillin were resistance with 60%and 70% respectively. Also the molecular variation for these isolates for virulence factors were detected and the characterization of bacteria was confirmed by checking 16SrRNA gene, after the specific primers were designed for each gene of virulence which included apH,flcB and lasB also specific primer for 16SrRNA was designed. The results showed that the characterization of bacteria was confirmed by 16SrRNA gene detection and sequence and the isolates contain the virulence genes had some polymorphism in comparison with those in NCBI. | ||
| Keywords | ||
| PCR; Antibiotic resistance; Pseudomonas aeruginosa | ||
| References | ||
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