abd Al-salam Obeed, D., Qais Abd, M., Mohamed Abdullah, E. (2015). The detection on the infection with the H.pylori by using molecular test (PCR ) by using the different genes and different sampling in the Al-Ramadi city. , 9(3), 177-184. doi: 10.37652/juaps.2015.127642
Dhuha abd Al-salam Obeed; Mohammed Qais Abd; Essam Mohamed Abdullah. "The detection on the infection with the H.pylori by using molecular test (PCR ) by using the different genes and different sampling in the Al-Ramadi city". , 9, 3, 2015, 177-184. doi: 10.37652/juaps.2015.127642
abd Al-salam Obeed, D., Qais Abd, M., Mohamed Abdullah, E. (2015). 'The detection on the infection with the H.pylori by using molecular test (PCR ) by using the different genes and different sampling in the Al-Ramadi city', , 9(3), pp. 177-184. doi: 10.37652/juaps.2015.127642
abd Al-salam Obeed, D., Qais Abd, M., Mohamed Abdullah, E. The detection on the infection with the H.pylori by using molecular test (PCR ) by using the different genes and different sampling in the Al-Ramadi city. , 2015; 9(3): 177-184. doi: 10.37652/juaps.2015.127642

The detection on the infection with the H.pylori by using molecular test (PCR ) by using the different genes and different sampling in the Al-Ramadi city

Journal of University of Anbar for Pure Science
Article 29, Volume 9, Issue 3, 2015, Pages 177-184    PDF (721.32 K)
Document Type: Research Paper
DOI: 10.37652/juaps.2015.127642
Authors
Dhuha abd Al-salam Obeed1; Mohammed Qais Abd* 1; Essam Mohamed Abdullah2
1University of Anbar - College of Science
2University of Anbar - College of Pharmacy
Abstract
The study aims at finding the infection of Helicobacter pylori by using the molecular test (polymerase chain reaction ) to finding the genes that most efficient in the diagnosis and the easiest sample in usage . the study collect samples from outpatients who customarily visit the medical sight unit > the collections of the sample include biopsies tissue , stool , and saliva from each patient , as well as , samples were collected from the control group (people who are not infection ) . After fiat the molecular test for biopsy tissue is most efficient when sensitivity of 86% and specificity of 100% for the different genes usage , and the molecular test for stool samples are the sensitivity and specificity 70 % and 100 % respectively , while the molecular test for saliva sampling are not found the positive result .
Keywords
pylori; PCR
References
1. Marshall B.J. ; and Warren , J.R., (1984). unidentified curved bacilli on gastric epithelium in active chronic gastritis. Journal Lancet; Vol. 1: pp. 1311-1315.

2. Hong, LU ; and Shu, Dong XIAO , (2014). New ideas for future studies of Helicobacter pylori. Journal of Digestive Diseases, Vol. 15: pp. 1-4.

3. Garza-Gonzalez, Elvira ; Perez-Perez, Guillermo Ignacio ; Maldonado-Garza , Héctor Jesús ; and Bosques-Padilla , Francisco Javier , (2014). A review of Helicobacter pylori diagnosis, treatment, and methods to detect eradication. journal World J Gastroenterol , Vol. 20 , No. 6 : pp. 1438-1449.

4. Atheron , John C.,(1998).H.pylori virulence factors. Journal , British medical Bulletin ; NO.1 : pp 105 - 120.

5. Engstrand , L. ; and Lindberg , M. , (2013). Helicobacter pylori and the gastric microbiota. Best Pract. Journal Res. Clin. Gastroenterol., Vol. 27: pp.39-45.

6. Blaser, M.J. (2005): An angered species in the stomach. Journal Sci. Am., Vol. 92: pp. 38-45.

7. Lydia ,Wroblewski E. ; Peek Jr. Richard M.; and Wilson Keith T., (2010). Helicobacter pylori and Gastric Cancer: Factors ThatModulate Disease Risk Journal Clin. Microbiol. Rev. , Vol.23 , No.4 : pp.713. DOI:10.1128/CMR.00011-10

8. Alessandro , Federico ; Gerarda, Gravina Antonietta ; Miranda, Agnese ; Loguercio, Carmela ; and Romano, Marco , (2014). Eradication of Helicobacter pylori infection: Which regimen first?.Journal World Gastroenterol ; Vol. 20, No.3: pp 665-672.

9. Zsikla, V. ; Hailemariam, S. ; and Baumann, M., (2006) Increased rate of Helicobacter pylori infection detected by PCR in biopsies with chronic gastritis. Journal The Am.J. of Surgical Pathology., Vol. 30 , No. 2 : pp. 242–248.

10. Fukuda , K. ; Kuroki , T. ; Tajima , Y. ; Tsuneoka m N. ; Kitajima , T. ; Matsuzaki , S. ; Furui , J. ; and Kanematsu , T. , (2002). Comparative analysis of Helicobacter DNAs and biliary pathology in patients with and without hepatobiliary cancer. Journal Carcinogenesis , Vol. 23 , pp. 1927 – 1931.

11. Bakir, Wasan A. , (2007). Immunological and Molecular Studies on Gastroduodenal Diseases Caused by Helicobacter pylori. University Anbar.

  1. 12.  Suerbaum ,S. ; and Michetti , P. , (2002). Helicobacter pylori infection. Jornal N Engl J Med, vol. 347: pp. 1175–1186.
13. Boyanova, L. ,(2011). Helicobacter pylori. Journal Caister Academic press.ISBN 978-1-904455-84-4.

  1. 14.  Kalaf , Elham Abdulhadi , (2013). Molecular Study for Detection of CagA Genotype of Helicobacter pylori from Endoscopic Biopsies of Iraqi Patients. University of Baghdad.
15. Sowjanya , Kanna ; Carla , Maradey-Romero; and Ronnie, Fass ,(2013). Diagnostic tests for Helicobacter pylori.Journal Castroenterology Endoscopy News.

16. Ibrahim, Nerman H. ; Gomaa, Azza Abdulazim ; Abu-Sief , Mohamed Ahmed ; Hifnawy , Tamer M. ; and Tohamy, Mervat Abd El-Baser , (2012). The use of different laboratory method in diagnosis of Helicobacter pylori infaction ; a comparative study. Jornal Life science , Vol 9, No. 4.

17. Wannmacher, Lennita , (2011). Review of the evidence for H. pylori treatment regimens.

18. Chey WD , Wong BC , 2007. American College of Gastroentrology guideline on the management of Helicobacter pylori infection. Am J Gastroenterol ; 102 (8) : 1808 – 1825

19. Rimbara, E. ; Sasatsu , M. ; and Graham , D.Y., (2013). PCR detection of Helicobacter pylori in clinical samples. Journal Methods Mol Biol, Vol. 943:pp. 279-287. [PMID: 23104297 DOI: 10.1007/978-1-60327-353- 4_19].

20. Whitmire , J.M.; and Merrell, D.S. , (2012). Successful culture techniques for Helicobacter species: verification of Helicobacter identity using 16SrRNA gene sequence analysis.Journal Methods J Mol Biol, Vol. 921: pp. 37-40. [PMID: 23015489 DOI: 10.1007/978-1-62703-005-2_6].

21. Dus, I. ; Dobosz, T. ; Manzin, A. ; Loi, G. ; Serra , C. ; and Radwan-Oczko, M., (2013). Role of PCR in Helicobacter pylori diagnostics and research--new approaches for study of coccoid and spiral : forms of the bacteria. Journal Postepy Hig Med Dosw (Online), Vol. 67: pp.261-268. [PMID: 23619225].

22. Owen, R.J. ,(2002). Molecular testing for antibiotic resistance in Helicobacter pylori. Journal Gut , Vol. 50: pp. 285-289. [PMID: 11839700].

23. Lawson, A.J. ; Elviss, N.C. ; and Owen, R.J. , (2005). Real time PCR detection and frequency of 16srDNA mutations associated with resistance and reduced susceptibility to tetracycline in Helicobacter pylori from England and Wales. Journal Antimicrob Chemother , Vol. 56, No. 2 : pp. 282 – 286.

24. de Francesco , V. ; Margiotta, M.; Zullo, A. ; and et.al., (2006). Primary clarithromycin resistance in Italy assessed on Helicobacter pylori DNA sequences by TaqMan real – time polymerase chain reaction. Journal Aliment Pharmacol Ther , Vol. 23, No. 3 : pp. 429 – 435.

  1. 25.  Schmitt , B.H. ; Regner, M. ; Mangold, K.A. ; Thomson, R.B.; and Kaul, K.L. , (2013). PCR detection of clarithromycin–susceptible and resistant Helicobacter pylori from formalin fixed , paraffin embedded gastric biopsies. Journal Mod Pathol , Vol. 26, No. 9 : pp. 1222 – 1227.
26. Ali , Sama Fakhri , (2013). Detection of Helicobacter pylori in saliva from some Iraqi patients in comparison with other methods. M.Sc. , University of Baghdad.

27. Pereira, Weendelly Nayara; Ferraz, Mariane Avante; Zabaglia, Luanna Munhoz; de Labio, Roger William; Orcini, Wilson Aparecido; Ximenez , Joao Paulo Bianchi ; Neto , Agostinho Caleman ; Payao ,Spencer Luiz Marques; and Rasmussen, Lucas Trevizani , (2014). Association among H. pylori virulence markers dupA, cagA and vacA in Brazilian patients Journal of Venomous Animals and Toxins including Tropical Diseases,Vol.20 : pp.1.

28. Ahmed , Khawaja Shakeel ; Madompoyil , Basheer ; Ghebremedhin , Anghesom Ambesajir ; Issac, John ; Ahi ,Janak Dular ; Khan , Aleem Ahmed ; Tiwari, Santosh Kumar , (2013). Studying the Importance of VacA Gene of Helicobacter pylori in Identifying the Pathogenicity of Strains by Comparing It with the Disease Status of the Subjects.Journal of Cancer Therapy, Vol. 4 : pp. 68-74.

29. Mishra , S. ; Singh, V.; Rao, G. ; and et.al., (2008) Detection of Helicobacter pylori in stool specimens: comparative evaluation of nested PCR and antigen detection. Journal Infect Genet E ,vol. 8: pp.815–819.

30. Wesolowska-Andersen , Agata ; Bahl ,Martin Iain ; Carvalh , Vera; Kristiansen , Karsten ; Sicheritz-Ponten , Thomas ; Gupta , Ramneek ; and Licht ,Tine Rask , (2014). Choice of bacterial DNA extraction method from fecal material influences community structure as evaluated by metagenomic analysis. Journal Microbiome , Vol. 2 : pp. 19.

31. Olivier, B. J. ; Bond, R. P. ; van Zyl, W. B. ; Delport, M. ; Slavik, T. ; Ziady, C. ; Terhaar, Sive. Droste, J. S. ; Lastovica, A.; and van der Merwe, S. W., (2006). Absence of Helicobacter pylori within the oral cavities of members of a healthy South African community. Journal Clin. Microbiol, Vol. 44: pp. 635–636.

32. Momtaz, Hassan ; Souod, Negar; Dabiri, Hossein; and Sarshar, Meysam , (2012). Study of Helicobacter pylori genotype status in saliva , dental plaques, stool and gastric biopsy samples. Journal World J Gastroenterol , Vol. 18 No.17 : pp. 2105-2111. doi:10.3748/wjg.v18. i17.2105.

33. Riggio, M. P. ; Lennon, A.; and Wray, D., (2000). Detection of Helicobacter pylori DNA in recurrent aphthous stomatitis tissue by PCR. Journal Oral Pathol. Med., Vol. 29: pp.507– 13.

34. Sushil, Kumar; Ashok, Kumar ;and Vinod, Kumar Dixit , (2010). Diversity in the cag pathogenicity island of Helicobacter pylori isolates in populations from North and South India. Journal of Medical Microbiology, Vol 59, pp. 32–40.

35. Chisholm , Stephanie A. ; Owen , Robert J. ; Teare , E. Louise ; and ; Saverymuttu , Seth , (2001). PCR-Based Diagnosis of Helicobacter pylori Infection and Real-Time Determination of Clarithromycin Resistance Directly from Human Gastric Biopsy Samples. Journal Clinical Microbiology , Vol. 39, No. 4 : p. 1217–1220.

36. Hussein, N.; Robinson, K. ; and Atherton, J. (2008). A study of age specific Helicobacter pylori seropositivity rates in Iraq.Journal Helicobacter, Vol. 13: pp.306- 307.

Statistics
Article View: 425
PDF Download: 288


Journal Management System. Powered by iJournalPro.com