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Isolation and identification of different diarrheagenic (DEC) Escherichia coli pathotypes from children under five years old in Baghdad | ||
| IRAQI JOURNALOF COMMUNITY MEDICINE | ||
| Article 1, Volume 28, Issue 3, 2015, Pages 126-132 | ||
| Author | ||
| Zena K. Khalil | ||
| Abstract | ||
| : Diarrheagenic Escherichia coli strains can be classified into six main pathotypes on the basis of their specific virulence properties, association with some serotypes, and different epidemiological and clinical features. The identification of DEC cannot be based only on cultural and biochemical criteria, since they are indistinguishable from the non-pathogenic E. coli and specific serotyping is not always correlated with pathogenicity. Since several virulence factors and DNA sequences of DEC have been identified, they can be determined by the presence of Materials and Methods: From June 2014 –September 2014 , one hundred thirty two stool samples were collected from diarrheal children admitted to the Pediatric Central Hospital in Baghdad and different diarrheagenic (DEC) Escherichia coli pathotypes isolated by using multiplex polymerase chain reaction , the susceptibility of isolated Escherichia coli to some selected antibiotics were identified by using disc diffusion method . In order to distinguish typical and atypical EPEC strains, these were tested for the presence of EAF plasmid. Results: The frequency of diarrheagenic E. coli was (38.6%) and the most frequently isolated pathotypes was EAEC (35.3%), followed by atypical EPEC (29.4%), ETEC (21.6%),and STEC (7.8%). EPEC+ STEC (3.9%) and typical EPEC was detected only in one sample (2%). The frequency of the pathotypes studied were (89%) occurred among children less than 2 years old. EAEC was mainly detected among children under the age of 1 year (61%) , EPEC in children aged younger than 2 years (87%) and ETEC in children older than 1 year (64%) . Among children with diarrhea who were positive for DEC, (80.4%) had watery diarrhea and (19.6%) had bloody diarrhea. Fever was the most commonly symptom reported in association with DEC (76.5%) followed by vomiting (19.5%) and dehydration (4%), and the highest sensitivity E. coli isolates was recorded for Cefotaxime (92%) followed by Ciprofloxacin (90%) and Gentamicin (82%), while the highest resistance was observed against Oxytetracycline (84%) and Doxycycline hydrochloride (80%) followed by Ampicillin (77%) and Co-trimoxazole (75%) . Conclusions: This study reveals that DEC strains contribute to the burden of children diarrheal diseases and EAEC is the most commonly identified DEC strain. The highest sensitivity E. coli isolates was recorded for Cefotaxime (92%) . | ||
| Keywords | ||
| Children; BAGHDAD CITY; diarrheagenic Escherichia coli | ||
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